2D NMR is extensively used in many different fields, and its potential for the study of complex biochemical or chemical mixtures has been widely demonstrated. 2D NMR gives the ability to resolve peaks that overlap in 1D spectra, while providing both structural and quantitative information. However, complex mixtures are often analysed in situations where the data acquisition time is a crucial limitation, due to an ongoing chemical reaction or a moving sample from a hyphenated technique, or to the high-throughput requirement associated with large sample collections. Among the great diversity of available fast 2D methods, ultrafast (or single-scan) 2D NMR is probably the most general and versatile approach for complex mixture analysis. Indeed, ultrafast NMR has undergone an impressive number of methodological developments that have helped turn it into an efficient analytical tool, and numerous applications to the analysis of mixtures have been reported. This review first summarizes the main concepts, features and practical limitations of ultrafast 2D NMR, as well as the methodological developments that improved its analytical potential. Then, a detailed description of the main applications of ultrafast 2D NMR to mixture analysis is given. The two major application fields of ultrafast 2D NMR are first covered, i.e., reaction/process monitoring and metabolomics. Then, the potential of ultrafast 2D NMR for the analysis of hyperpolarized mixtures is described, as well as recent developments in oriented media. This review focuses on high-resolution liquid-state 2D experiments (including benchtop NMR) that include at least one spectroscopic dimension (i.e., 2D spectroscopy and DOSY) but does not cover in depth applications without spectral resolution and/or in inhomogeneous fields.

二维核磁共振广泛应用于许多不同领域，其在研究复杂生化或化学混合物方面的潜力已得到广泛证明。二维 NMR 能够解析一维光谱中重叠的峰，同时提供结构和定量信息。然而，由于正在进行的化学反应或来自联用技术的移动样品，或者与大量样品收集相关的高通量要求，在数据采集时间成为关键限制的情况下，通常会分析复杂的混合物。在众多可用的快速二维方法中，超快（或单次扫描）二维核磁共振可能是复杂混合物分析中最通用、最通用的方法。的确，超快 NMR 经历了大量的方法学发展，使其成为一种有效的分析工具，并且已经报道了许多在混合物分析中的应用。本综述首先总结了超快 2D NMR 的主要概念、特征和实际局限性，以及提高其分析潜力的方法学发展。然后，详细描述了超快二维核磁共振在混合物分析中的主要应用。首先涵盖了超快二维核磁共振的两大应用领域，以及提高其分析潜力的方法学发展。然后，详细描述了超快二维核磁共振在混合物分析中的主要应用。首先涵盖了超快二维核磁共振的两大应用领域，以及提高其分析潜力的方法学发展。然后，详细描述了超快二维核磁共振在混合物分析中的主要应用。首先涵盖了超快二维核磁共振的两大应用领域，即，反应/过程监测和代谢组学。然后，描述了超快 2D NMR 在分析超极化混合物方面的潜力，以及定向介质的最新发展。本综述侧重于高分辨率液态 2D 实验（包括台式 NMR），这些实验至少包括一个光谱维度（即2D 光谱和 DOSY），但不涵盖没有光谱分辨率和/或非均匀场的深度应用。