Bone homeostasis is maintained by a balance between bone formation by osteoblasts and bone resorption by osteoclasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Our previous studies showed the plant-derived natural polysaccharide (Polygonatum sibiricum polysaccharide or PSP) had significant anti-ovariectomy (OVX)-induced osteoporosis effects in vivo, but the mechanisms of PSP's anti-osteoporosis effect remains unclear. In this study, we assessed PSP's effect on the generation of osteoblast and osteoclast in vitro. This study showed that PSP promoted the osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) without affecting BMPs signaling pathway. This effect was due to the increased nuclear accumulation of β-catenin, resulting in a higher expression of osteoblast-related genes. Furthermore, the study showed PSP could inhibit the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and exert prophylatic protection against LPS-induced osteolysis in vivo. This effect was also related to the increased nuclear accumulation of β-catenin, resulting in the decreased expression of osteoclast-related genes. In conclusion, our results showed that PSP effectively promoted the osteogenic differentiation of mouse BMSCs and suppressed osteoclastogenesis; therefore, it could be used to treat osteoporosis.

中文翻译：

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玉竹多糖通过促进成骨细胞的形成并通过Wnt /β-catenin信号传导通路阻断破骨细胞形成，从而抑制骨质疏松症。

骨稳态通过成骨细胞形成的骨与破骨细胞吸收的骨之间的平衡来维持。当破骨细胞活性超过成骨细胞活性时，就会发生骨质疏松。我们以前的研究表明，植物来源的天然多糖（玉竹多糖或PSP）在体内具有明显的抗卵巢切除术（OVX）诱导的骨质疏松作用，但PSP的抗骨质疏松作用机理尚不清楚。在这项研究中，我们评估了PSP在体外对成骨细胞和破骨细胞生成的影响。这项研究表明，PSP可以促进小鼠骨髓基质细胞（BMSCs）的成骨分化，而不会影响BMPs信号通路。这种作用是由于β-catenin的核积累增加，导致成骨细胞相关基因的表达更高。此外，该研究表明，PSP可以抑制核因子-κB配体（RANKL）诱导的破骨细胞生成的受体激活剂，并在体内对LPS诱导的溶骨起到预防作用。该作用还与β-连环蛋白的核积累增加有关，导致破骨细胞相关基因的表达降低。总之，我们的结果表明，PSP可有效促进小鼠骨髓间充质干细胞的成骨分化并抑制破骨细胞的生成。因此，它可以用于治疗骨质疏松症。导致破骨细胞相关基因的表达下降。总之，我们的结果表明，PSP可有效促进小鼠骨髓间充质干细胞的成骨分化并抑制破骨细胞的生成。因此，它可以用于治疗骨质疏松症。导致破骨细胞相关基因的表达下降。总之，我们的结果表明，PSP可有效促进小鼠骨髓间充质干细胞的成骨分化并抑制破骨细胞的生成。因此，它可以用于治疗骨质疏松症。