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Synergistic interactions between artocarpin-rich extract, lawsone methyl ether and ampicillin on anti-MRSA and their antibiofilm formation
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2022-01-31 , DOI: 10.1111/lam.13662 R R Bazmi 1, 2 , P Panichayupakaranant 1, 3
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2022-01-31 , DOI: 10.1111/lam.13662 R R Bazmi 1, 2 , P Panichayupakaranant 1, 3
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Artocarpin-rich extract (ARE) was prepared using a green technology and standardized to contain 49·6% w/w artocarpin, while lawsone methyl ether was prepared using a green semi-synthesis. ARE, LME and ampicillin exhibited weak anti-MRSA activity with the MICs of 31·2–62·5 µg/ml. Based on the checkerboard assay, the synergistic interaction between ARE (0·03 µg/ml) and LME (0·49 µg/ml) against four MRSA isolates were observed with the fractional inhibitory concentration index (FICI) value of 0·008, while those of ARE (1·95–7·81 µg/ml) and ampicillin (0·49 µg/ml) as well as LME (0·49–1·95 µg/ml) and ampicillin (0·49 µg/ml) were 0·016–0·257. The time kill confirmed the synergistic interactions against MRSA with different degrees. The combination of ARE and LME as well as its combinations with ampicillin altered the membrane permeability of MRSA, which led to release of the intracellular materials. In addition, each compound inhibited the biofilm formation of standard MRSA (DMST 20654) and the clinical isolate (MRSA 1096). These findings suggested that cocktails containing ARE and LME might be used to overcome problems associated with MRSA. Additionally, the results implied that combination of either ARE or LME with available conventional antibiotic agents might be effective in countering these perilous pathogens.
中文翻译：
富含artocarpin的提取物、指甲花甲醚和氨苄青霉素对抗MRSA及其抗生物膜形成的协同作用
富含青蒿素的提取物 (ARE) 采用绿色技术制备，标准化为含有 49·6% w/w 的青蒿素，而指甲花松甲醚则使用绿色半合成法制备。ARE、LME 和氨苄青霉素表现出较弱的抗 MRSA 活性，MIC 为 31·2–62·5 µg /ml。基于棋盘分析，观察到 ARE (0·03 µ g/ml) 和 LME (0·49 µ g/ml) 对四种 MRSA 分离株的协同作用，分数抑制浓度指数 (FICI) 值为 0· 008，而 ARE (1·95–7·81 µ g/ml) 和氨苄青霉素 (0·49 µ g/ml) 以及 LME (0·49–1·95 µ g/ml) 和氨苄青霉素 ( 0·49 µg/ml) 为 0·016–0·257。时间杀伤证实了对 MRSA 不同程度的协同作用。ARE 和 LME 的组合以及其与氨苄青霉素的组合改变了 MRSA 的膜通透性，从而导致细胞内物质的释放。此外，每种化合物都抑制标准 MRSA (DMST 20654) 和临床分离物 (MRSA 1096) 的生物膜形成。这些发现表明，含有 ARE 和 LME 的混合物可用于克服与 MRSA 相关的问题。此外，结果表明 ARE 或 LME 与现有的常规抗生素药物的组合可能有效对抗这些危险的病原体。
更新日期：2022-01-31
中文翻译：
富含artocarpin的提取物、指甲花甲醚和氨苄青霉素对抗MRSA及其抗生物膜形成的协同作用
富含青蒿素的提取物 (ARE) 采用绿色技术制备，标准化为含有 49·6% w/w 的青蒿素，而指甲花松甲醚则使用绿色半合成法制备。ARE、LME 和氨苄青霉素表现出较弱的抗 MRSA 活性，MIC 为 31·2–62·5 µg /ml。基于棋盘分析，观察到 ARE (0·03 µ g/ml) 和 LME (0·49 µ g/ml) 对四种 MRSA 分离株的协同作用，分数抑制浓度指数 (FICI) 值为 0· 008，而 ARE (1·95–7·81 µ g/ml) 和氨苄青霉素 (0·49 µ g/ml) 以及 LME (0·49–1·95 µ g/ml) 和氨苄青霉素 ( 0·49 µg/ml) 为 0·016–0·257。时间杀伤证实了对 MRSA 不同程度的协同作用。ARE 和 LME 的组合以及其与氨苄青霉素的组合改变了 MRSA 的膜通透性，从而导致细胞内物质的释放。此外，每种化合物都抑制标准 MRSA (DMST 20654) 和临床分离物 (MRSA 1096) 的生物膜形成。这些发现表明，含有 ARE 和 LME 的混合物可用于克服与 MRSA 相关的问题。此外，结果表明 ARE 或 LME 与现有的常规抗生素药物的组合可能有效对抗这些危险的病原体。
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