Serum Metabolic Fingerprints on Bowl-Shaped Submicroreactor Chip for Chemotherapy Monitoring,ACS Nano

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Serum Metabolic Fingerprints on Bowl-Shaped Submicroreactor Chip for Chemotherapy Monitoring
ACS Nano ( IF 15.8 ) Pub Date : 2022-01-31 , DOI: 10.1021/acsnano.1c09864
Xia Yin ₁ , Jing Yang _{1,

2} , Mengji Zhang _{1,

2} , Xinyao Wang ₃ , Wei Xu _{1,

2} , Cameron-Alexander H Price _{4,

5} , Lin Huang _{1,

2} , Wanshan Liu _{1,

2} , Haiyang Su _{1,

2} , Wenjing Wang ₁ , Hongyu Chen ₃ , Guangjin Hou ₃ , Mark Walker ₆ , Ying Zhou ₇ , Zhen Shen ₇ , Jian Liu _{3,

8} , Kun Qian _{1,

2} , Wen Di ₁

Affiliation

State Key Laboratory for Oncogenes and Related Genes, Shanghai Key Laboratory of Gynecologic Oncology, Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P.R. China.
School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China.
State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, P.R. China.
The University of Manchester at Harwell, Diamond Light Source, Didcot, Oxfordshire OX11 0DE, U.K.
UK Catalysis Hub, Research Complex at Harwell, Rutherford Appleton Laboratories, Harwell Campus, Didcot, Oxfordshire OX11 0FA, U.K.
Department of Obstetrics and Gynecology, University of Ottawa, Ottawa, Ontario ON K1H 8L6, Canada.
Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Auhui 230001, P.R. China.
DICP-Surrey Joint Centre for Future Materials, Department of Chemical and Process Engineering, and Advanced Technology Institute, University of Surrey, Guilford, Surrey GU2 7XH, U.K.

Chemotherapy is a primary cancer treatment strategy, the monitoring of which is critical to enhancing the survival rate and quality of life of cancer patients. However, current chemotherapy monitoring mainly relies on imaging tools with inefficient sensitivity and radiation invasiveness. Herein, we develop the bowl-shaped submicroreactor chip of Au-loaded 3-aminophenol formaldehyde resin (denoted as APF-bowl&Au) with a specifically designed structure and Au loading content. The obtained APF-bowl&Au, used as the matrix of laser desorption/ionization mass spectrometry (LDI MS), possesses an enhanced localized electromagnetic field for strengthened small metabolite detection. The APF-bowl&Au enables the extraction of serum metabolic fingerprints (SMFs), and machine learning of the SMFs achieves chemotherapy monitoring of ovarian cancer with area-under-the-curve (AUC) of 0.81–0.98. Furthermore, a serum metabolic biomarker panel is preliminarily identified, exhibiting gradual changes as the chemotherapy cycles proceed. This work provides insights into the development of nanochips and contributes to a universal detection platform for chemotherapy monitoring.

中文翻译：

用于化疗监测的碗形亚微反应器芯片上的血清代谢指纹

化疗是一种主要的癌症治疗策略，对其进行监测对于提高癌症患者的生存率和生活质量至关重要。然而，目前的化疗监测主要依赖于灵敏度低且辐射侵袭性低的成像工具。在此，我们开发了具有专门设计的结构和 Au 负载量的负载 Au 的 3-氨基苯酚甲醛树脂（表示为 APF-bowl&Au）的碗形亚微反应器芯片。获得的 APF-bowl&Au 用作激光解吸/电离质谱 (LDI MS) 的基质，具有增强的局部电磁场，可用于增强小代谢物的检测。APF-bowl&Au 能够提取血清代谢指纹 (SMF)，SMF 的机器学习实现了对卵巢癌的化疗监测，曲线下面积 (AUC) 为 0.81-0.98。此外，初步确定了血清代谢生物标志物组，随着化疗周期的进行呈现逐渐变化。这项工作为纳米芯片的发展提供了见解，并有助于建立一个用于化疗监测的通用检测平台。

更新日期：2022-01-31

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