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Photoacoustic Carbon Nanotubes Embedded Silk Scaffolds for Neural Stimulation and Regeneration
ACS Nano ( IF 15.8 ) Pub Date : 2022-01-31 , DOI: 10.1021/acsnano.1c08491
Nan Zheng , Vincent Fitzpatrick 1 , Ran Cheng , Linli Shi , David L Kaplan 1 , Chen Yang
Affiliation  

Neural interfaces using biocompatible scaffolds provide crucial properties, such as cell adhesion, structural support, and mass transport, for the functional repair of nerve injuries and neurodegenerative diseases. Neural stimulation has also been found to be effective in promoting neural regeneration. This work provides a generalized strategy to integrate photoacoustic (PA) neural stimulation into hydrogel scaffolds using a nanocomposite hydrogel approach. Specifically, polyethylene glycol (PEG)-functionalized carbon nanotubes (CNT), highly efficient photoacoustic agents, are embedded into silk fibroin to form biocompatible and soft photoacoustic materials. We show that these photoacoustic functional scaffolds enable nongenetic activation of neurons with a spatial precision defined by the area of light illumination, promoting neuron regeneration. These CNT/silk scaffolds offered reliable and repeatable photoacoustic neural stimulation, and 94% of photoacoustic-stimulated neurons exhibit a fluorescence change larger than 10% in calcium imaging in the light-illuminated area. The on-demand photoacoustic stimulation increased neurite outgrowth by 1.74-fold in a rat dorsal root ganglion model, when compared to the unstimulated group. We also confirmed that promoted neurite outgrowth by photoacoustic stimulation is associated with an increased concentration of neurotrophic factor (BDNF). As a multifunctional neural scaffold, CNT/silk scaffolds demonstrated nongenetic PA neural stimulation functions and promoted neurite outgrowth, providing an additional method for nonpharmacological neural regeneration.

中文翻译:

用于神经刺激和再生的光声碳纳米管嵌入丝支架

使用生物相容性支架的神经界面为神经损伤和神经退行性疾病的功能修复提供了关键特性,例如细胞粘附、结构支撑和传质。还发现神经刺激可有效促进神经再生。这项工作提供了一种通用策略,使用纳米复合水凝胶方法将光声 (PA) 神经刺激整合到水凝胶支架中。具体而言,将聚乙二醇 (PEG) 功能化的碳纳米管 (CNT) 这种高效光声剂嵌入丝素蛋白中,形成具有生物相容性的软光声材料。我们表明,这些光声功能支架能够以由光照区域定义的空间精度激活神经元的非遗传激活,从而促进神经元再生。这些碳纳米管/丝支架提供了可靠且可重复的光声神经刺激,并且 94% 的光声刺激神经元在光照区域的钙成像中表现出大于 10% 的荧光变化。与未受刺激的组相比,按需光声刺激使大鼠背根神经节模型的神经突生长增加了 1.74 倍。我们还证实,光声刺激促进的神经突生长与神经营养因子 (BDNF) 浓度增加有关。作为一种多功能神经支架,CNT/丝支架展示了非遗传性 PA 神经刺激功能并促进了神经突生长,为非药物神经再生提供了一种额外的方法。94% 的光声刺激神经元在光照区域的钙成像中表现出大于 10% 的荧光变化。与未受刺激的组相比,按需光声刺激使大鼠背根神经节模型的神经突生长增加了 1.74 倍。我们还证实,光声刺激促进的神经突生长与神经营养因子 (BDNF) 浓度增加有关。作为一种多功能神经支架,CNT/丝支架展示了非遗传性 PA 神经刺激功能并促进了神经突生长,为非药物神经再生提供了一种额外的方法。94% 的光声刺激神经元在光照区域的钙成像中表现出大于 10% 的荧光变化。与未受刺激的组相比,按需光声刺激使大鼠背根神经节模型的神经突生长增加了 1.74 倍。我们还证实,光声刺激促进的神经突生长与神经营养因子 (BDNF) 浓度增加有关。作为一种多功能神经支架,CNT/丝支架展示了非遗传性 PA 神经刺激功能并促进了神经突生长,为非药物神经再生提供了一种额外的方法。与未受刺激的组相比,按需光声刺激使大鼠背根神经节模型的神经突生长增加了 1.74 倍。我们还证实,光声刺激促进的神经突生长与神经营养因子 (BDNF) 浓度增加有关。作为一种多功能神经支架,CNT/丝支架展示了非遗传性 PA 神经刺激功能并促进了神经突生长,为非药物神经再生提供了一种额外的方法。与未受刺激的组相比,按需光声刺激使大鼠背根神经节模型的神经突生长增加了 1.74 倍。我们还证实,光声刺激促进的神经突生长与神经营养因子 (BDNF) 浓度增加有关。作为一种多功能神经支架,CNT/丝支架展示了非遗传性 PA 神经刺激功能并促进了神经突生长,为非药物神经再生提供了一种额外的方法。
更新日期:2022-01-31
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