模拟骨软骨组织的组成和结构以制造用于修复骨软骨缺损的异质三层支架,ACS Applied Bio Materials

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模拟骨软骨组织的组成和结构以制造用于修复骨软骨缺损的异质三层支架
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2022-01-31 , DOI: 10.1021/acsabm.1c01152
Hongmei Zhou ₁ , Lun Yuan ₁ , Zhilang Xu ₁ , Xueling Yi ₁ , Xiao Wu ₁ , Changdao Mu ₁ , Liming Ge ₁ , Defu Li ₁

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通过模拟骨软骨组织的透明软骨、钙化软骨和软骨下骨的生化组成和结构，制备了异质三层支架，用于修复骨软骨缺损。透明软骨层由胶原蛋白 I (50.0 wt%) 和透明质酸钠 (50.0 wt%) 组成。钙化软骨层和软骨下骨层由不同比例的胶原蛋白I、透明质酸钠和纳米羟基磷灰石组成。N-羟基琥珀酰亚胺/ N- (3-二甲基氨基丙基) -N'-乙基碳二亚胺盐酸盐用于介导胶原蛋白的胺基与透明质酸钠的羧基的交联反应。透明软骨层和钙化软骨层设计为致密结构，而软骨下骨层通过调节交联度设计为相对松散的结构。支架呈现出均匀且相互连接的多孔结构，具有超过85%的高孔隙率，有利于细胞粘附和增殖。由于交联，支架在降解 30 天后可以保持在 50-75%，为骨软骨组织的再生提供了足够的时间。尤其是透明软骨层和钙化软骨层更倾向于诱导软骨细胞的增殖，而软骨下骨层更有利于成骨细胞的增殖。总之，异质多层支架可以作为骨软骨重建的植入材料。

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Mimicking the Composition and Structure of the Osteochondral Tissue to Fabricate a Heterogeneous Three-Layer Scaffold for the Repair of Osteochondral Defects

Heterogeneous three-layer scaffolds were fabricated by mimicking the biochemical composition and structure of the hyaline cartilage, calcified cartilage, and subchondral bone of the osteochondral tissue for the repair of osteochondral defects. The hyaline cartilage layer was composed of collagen I (50.0 wt %) and sodium hyaluronate (50.0 wt %). The calcified cartilage layer and subchondral bone layer were composed of collagen I, sodium hyaluronate, and nanohydroxyapatite with different proportions. N-Hydroxysuccinimide/N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride was used to mediate the crosslinking reaction of the amine groups of collagen with carboxyl groups of sodium hyaluronate. The hyaline cartilage layer and calcified cartilage layer were designed as dense structures, while the subchondral bone layer was designed as a relatively loose structure by adjusting the crosslinking degree. The scaffolds displayed a uniform and interconnected porous structure and possessed a high porosity over 85%, which were conducive to cellular adhesion and proliferation. The scaffolds could remain at 50–75% after 30 days of degradation owing to crosslinking, providing enough time for the regeneration of the osteochondral tissue. Especially, the hyaline cartilage layer and calcified cartilage layer preferred to induce the proliferation of chondrocytes, while the subchondral bone layer was more conducive to the proliferation of osteoblasts. In conclusion, the heterogeneous multilayer scaffolds could serve as implant materials for osteochondral reconstruction.

更新日期：2022-01-31

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