In this study, we successfully developed a new multifunctional chemosensor 4′,4‴-(2,2-diphenylethene-1,1-diyl)bis(N,N-bis (pyridin-2-ylmethyl)-[1,1′-biphenyl]-4-amine (T-D), based on the cooperation of tetraphenylethylene and di-(2-picolyl)amine (DPA), for sensitive, selective, and quick detection of Cu²⁺, PO₄³⁻ and pesticide glyphosate in aqueous solution. In our research, we made full use of the Cu²⁺-indicator displacement strategy to achieve the construction of an “on-off-on” fluorescent switch platform. Due to the presence of the DPA moiety in T-D, Cu²⁺ could be captured quickly to form the complex T-D-Cu and caused fluorescence quenching. With the addition of PO₄³⁻ and glyphosate, the system could rapidly restore the fluorescence by squeezing Cu²⁺ from T-D-Cu and blocking the photo induced electron transfer (PET) process to display the aggregation-induced emission. To demonstrate the possibility of practical applications, we detected PO₄³⁻ and glyphosate in spiked real samples. The detection limit for PO₄³⁻ and glyphosate reached 19 nM and 25 nM, respectively. Furthermore, test strips using T-D-Cu solution simplified the detection process of glyphosate. More importantly, the sensor could be used in visual semi-quantitative determination of PO₄³⁻ concentrations in both living cells and living zebra fish. Therefore, the chemosensor presented here will not only be a powerful tool for the detection of phosphate anions in aqueous solution and biological systems, but also provides a new template for the design of other multifunctional chemosensors.

在本研究中，我们成功开发了一种新型多功能化学传感器 4′,4‴-(2,2-diphenylethene-1,1-diyl)bis( N , N -bis (pyridin-2-ylmethyl)-[1,1′ -联苯]-4-胺 ( TD )，基于四苯基乙烯和二-(2-吡啶基)胺 (DPA) 的协同作用，用于对 Cu ²⁺、PO ₄ ^3-和农药草甘膦的灵敏、选择性和快速检测在水溶液中。在我们的研究中，我们充分利用Cu ²⁺指示剂置换策略，实现了“on-off-on”荧光开关平台的构建。由于TD中存在DPA部分，Cu ²⁺可以快速捕获形成复合体TD-Cu并引起荧光猝灭。通过添加PO ₄ ^3-和草甘膦，该系统可以通过从TD-Cu中挤压Cu ²⁺并阻断光诱导电子转移（PET）过程来快速恢复荧光以显示聚集诱导的发射。为了证明实际应用的可能性，我们在加标的实际样品中检测到 PO ₄ ^{3-和草甘膦。}_{PO 4} ^3-和草甘膦的检测限分别达到 19 nM 和 25 nM。此外，使用TD-Cu 的试纸解决方案简化了草甘膦的检测过程。更重要的是，该传感器可用于活细胞和活斑马鱼中PO ₄ ^{3-浓度的视觉半定量测定。}因此，这里介绍的化学传感器不仅将成为检测水溶液和生物系统中磷酸根阴离子的有力工具，而且为其他多功能化学传感器的设计提供了新的模板。