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Genome-wide identification, expression pattern and subcellular localization analysis of the JAZ gene family in Toona ciliata
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2022-01-25 , DOI: 10.1016/j.indcrop.2022.114582
Huiyun Song 1, 2 , Zhihao Duan 1, 2 , Zhi Wang 1, 2 , Yue Li 1, 2 , Yueyang Wang 1, 2 , Chunmei Li 1, 2 , Wenmai Mao 1, 2 , Qingmin Que 1, 2 , Xiaoyang Chen 3 , Pei Li 1, 2
Affiliation  

The jasmonate ZIM⁃domain (JAZ) is an inhibitor of the jasmonic acid (JA) signal transduction pathway, which plays a vital role in regulating plant growth and development and resisting diseases and insect pests. In this study, bioinformatics methods were used to identify the JAZ gene family from the whole genome of Toona ciliata and the gene characteristics, subcellular location, phylogenetic evolution, promoter cis-elements, and expression patterns were systematically analyzed. A total of 13 TciJAZs gene family members with complete TIFY and Jas domains were identified that were unevenly distributed on 9 chromosomes, encoding 134–400 amino acids, among which the molecular weights (MWs) was 15.75–42.29 kDa. Phylogenetic analysis showed that the TciJAZ gene family could be divided into five subfamilies (Group Ⅰ-Ⅴ) that have close relationships with Citrus clementina, and most TciJAZ family members contain light response and ABA response elements. The transcription factor (TF) families ERF, bZIP, and TCP play many roles with the JAZ family. Subcellular localization results shows that all TciJAZs genes expressed and function in nucleus. RT-qPCR results showed that TciJAZs are mainly expressed in flowers, mature leaves and tender stems. Under MeJA stress, all TciJAZs responded to varying degrees, among which TciJAZ3/4/5/6/7 had a prompt and strong response. Under Hypsipyla robusta stress, TciJAZ1/3/11 expression in leaves and tender stems was significantly upregulated, which may have an anti-insect function. This study provides a basis for further research on TciJAZ genes function and regulatory mechanism in the future and lays the foundation for anti-insect gene selection and insect-resistant breeding of T. ciliata.



中文翻译:

香椿JAZ基因家族全基因组鉴定、表达模式及亚细胞定位分析

茉莉酸ZIM-结构域(JAZ)是茉莉酸(JA)信号转导通路的抑制剂,在调节植物生长发育和抵抗病虫害方面发挥着至关重要的作用。本研究采用生物信息学方法从香椿全基因组中鉴定出JAZ基因家族,系统分析了其基因特征、亚细胞定位、系统进化、启动子顺式元件和表达模式。共鉴定出13个具有完整TIFY和Jas结构域的TciJAZs基因家族成员,它们不均匀分布在9条染色体上,编码134-400个氨基酸,其中分子量(MWs)为15.75-42.29 kDa。系统发育分析表明,TciJAZ基因家族可分为与柑桔亲缘关系较近的5个亚家族(Ⅰ-Ⅴ组),大部分TciJAZ家族成员含有光响应和ABA响应元件。转录因子 (TF) 家族 ERF、bZIP 和 TCP 在 JAZ 家族中发挥着许多作用。亚细胞定位结果显示所有TciJAZs基因均在细胞核中表达和发挥作用。RT-qPCR结果显示TciJAZs主要在花、成熟叶和嫩茎中表达。在MeJA压力下,所有TciJAZs都有不同程度的反应,其中TciJAZ3/4/5/6/7反应迅速而强烈。在Hypsipyla robusta胁迫下,TciJAZ1/3/11在叶片和嫩茎中的表达显着上调,可能具有抗虫功能。该研究为今后进一步研究TciJAZ基因的功能和调控机制奠定了基础,为纤毛虫的抗虫基因选择和抗虫育种奠定了基础

更新日期:2022-01-25
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