Nicotine is a harmful pollutant mainly from the waste of tobacco factories. It is necessary to remove nicotine via high efficient strategies such as bioremediation. So far, an increasing number of nicotine degrading strains have been isolated. However, their degrading efficiency and tolerance to high content nicotine is still not high enough for application in real environment. Thus, the modification of nicotine metabolism pathway is obligated and requires comprehensive molecular insights into whole cell metabolism of nicotine degrading strains. Obviously, the development of multi-omics technology has accelerated the mechanism study on microbial degradation of nicotine and supplied more novel strategy of strains modification. So far, three pathways of nicotine degradation, pyridine pathway, pyrrolidine pathway, and the variant of pyridine and pyrrolidine pathway (VPP pathway), have been clearly identified in bacteria. Muti-omics analysis further revealed specific genome architecture, regulation mechanism, and specific genes or enzymes of three pathways, in different strains. Especially, muti-omics analysis revealed that functional modules coexisted in different genome loci and played additional roles on enhanced degradation efficiency in bacteria. Based on the above discovery, genomic editing strategy becomes more feasible to greatly improve bacterial degrading efficiency of nicotine.

尼古丁是一种有害污染物，主要来自烟草厂的废物。有必要通过生物修复等高效策略去除尼古丁。迄今为止，已分离出越来越多的尼古丁降解菌株。然而，它们的降解效率和对高含量尼古丁的耐受性仍然不足以在实际环境中应用。因此，对尼古丁代谢途径的改造是必须的，并且需要对尼古丁降解菌株的全细胞代谢进行全面的分子洞察。显然，多组学技术的发展加速了尼古丁微生物降解机制的研究，为菌种修饰提供了更新颖的策略。到目前为止，尼古丁降解的三种途径，吡啶途径，吡咯烷途径，以及吡啶和吡咯烷途径（VPP途径）的变体，已在细菌中明确鉴定。多组学分析进一步揭示了不同菌株中特定的基因组结构、调控机制以及三种途径的特定基因或酶。特别是，多组学分析表明，功能模块共存于不同的基因组位点，并在提高细菌降解效率方面发挥了额外的作用。基于以上发现，基因组编辑策略变得更加可行，可以大大提高尼古丁的细菌降解效率。多组学分析表明，功能模块共存于不同的基因组位点，并在提高细菌降解效率方面发挥了额外的作用。基于以上发现，基因组编辑策略变得更加可行，可以大大提高尼古丁的细菌降解效率。多组学分析表明，功能模块共存于不同的基因组位点，并在提高细菌降解效率方面发挥了额外的作用。基于以上发现，基因组编辑策略变得更加可行，可以大大提高尼古丁的细菌降解效率。