Synthesis of a Single-Stranded DNA Aptamer Modified Near-infrared, Water-Soluble Fluorophore for Lung Cancer Cell Imaging,ChemistrySelect

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Synthesis of a Single-Stranded DNA Aptamer Modified Near-infrared, Water-Soluble Fluorophore for Lung Cancer Cell Imaging
ChemistrySelect ( IF 1.9 ) Pub Date : 2022-01-24 , DOI: 10.1002/slct.202103821
Xiaoyan He _{1,

2} , Hui Li ₃ , Sisi Liu ₃ , Yue Li ₁ , Xiangde Lin ₃ , Haoyang Zheng _{1,

2} , Zhaoli Zhou _{1,

2} , Dongdong Zeng _{1,

3}

Affiliation

In this work, two polyethylene glycol functionalized water soluble aza-BODIPY derivatives (a mono cycloaddition product 1PEG-BOD and a double cycloaddition product 2PEG-BOD) were synthesized through a cycloaddition click reaction between aza-BODIPY and N₃-PEG₂₀₀₀-COOH. The fluorescence quantum yield of 2PEG-BOD was 20 times higher than 1PEG-BOD in water. The attachment of a A549 lung cancer cell targeting aptamer with 2PEG-BOD afforded APT-PEG-BOD, which showed higher affinity to A549 lung cancer cell than 2PEG-BOD confirming by in vitro cell imaging experiment

中文翻译：

用于肺癌细胞成像的单链 DNA 适配体修饰的近红外水溶性荧光团的合成

_{在这项工作中，通过aza-BODIPY和N 3} -PEG ₂₀₀₀ -COOH之间的环加成点击反应合成了两种聚乙二醇功能化的水溶性aza-BODIPY衍生物（单环加成产物1PEG- BOD和双环加成产物2PEG- BOD ）。 . 2PEG- BOD在水中的荧光量子产率是1PEG- BOD的20倍。A549肺癌细胞靶向适体与2PEG- BOD的结合提供了APT - PEG - BOD ，与2PEG- BOD相比对A549肺癌细胞的亲和力更高通过体外细胞成像实验证实

更新日期：2022-01-24

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