In this study, the adsorbable SERS substrate was successfully fabricated on polydimethylsiloxane (PDMS) with the nanometer-sized roughness existing in a micrometer-sized roughness (nano-in-micro rough) structure which was completely transferred from the laser engraving aluminum (Al) mold by a replication process. Then the silver nanoparticle (Ag NPs) was deposited by magnetron sputtering. The physical morphology of the obtained samples was characterized by a field emission scanning electron microscope (FE-SEM). The ability for direct adsorb trace detection of the methylene blue (MB) on the glass slide surface was investigated. From the results, the SERS signal of the prepared adsorbable SERS substrates was influenced by the topography of the nano-in-micro rough feature and Ag NPs. It was found that combined nano-in-micro rough and microgroove structures exhibit high sensitivity of MB (10⁻⁵ M). In addition, the optimum adsorbable SERS substrate was then applied in practical application by adsorbing the methamphetamine (MAP) residues on the glass slide surface with a limit of 10⁻² M, which could be expected to be an on-site screening platform for drugs detection.

本研究成功地在聚二甲基硅氧烷 (PDMS) 上制备了可吸附的 SERS 基底，其纳米级粗糙度存在于完全从激光雕刻铝 (Al) 转移而来的微米级粗糙度（nano-in-micro rough）结构中。通过复制过程模具。然后通过磁控溅射沉积银纳米颗粒（Ag NPs）。通过场发射扫描电子显微镜（FE-SEM）对所得样品的物理形态进行表征。研究了直接吸附痕量检测载玻片表面上的亚甲蓝 (MB) 的能力。结果表明，制备的可吸附 SERS 基板的 SERS 信号受纳米微米粗糙特征和 Ag NPs 形貌的影响。^-5  M)。此外，通过将甲基苯丙胺（MAP）残留物吸附在载玻片表面，限制为10 ^-2  M ，将最佳的可吸附SERS基底应用于实际应用，有望成为药物的现场筛选平台。检测。