Journal of Proteomics ( IF 2.8 ) Pub Date : 2022-01-19 , DOI: 10.1016/j.jprot.2022.104485 Yasunori Sugiyama 1 , Yuuki Uezato 1
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Protein kinases regulate almost all biological processes including cell proliferation, differentiation, apoptosis, and gene expression. Dysregulation of protein phosphorylation caused by abnormal activity and expression of protein kinases results in the onset of various diseases such as cancer and metabolic syndromes. The activities of a large number of protein kinases are regulated by phosphorylation. Therefore, analysis of the phosphorylation status of protein kinases is important for elucidation of biological phenomena and the pathogenesis of diseases. To investigate protein phosphorylation, phosphate-binding tag molecule “Phos-tag” was developed. In addition, various techniques and tools using Phos-tag such as Phos-tag SDS-PAGE, have been developed for analysis and profiling of protein phosphorylation. Here, we describe the methods and analytical techniques that use Phos-tag for investigation of protein kinase phosphorylation and the applications of phosphorylation analysis.
Significance
Protein kinases play pivotal roles in regulating many biological processes and pathogenesis of diseases. Determination of phosphorylation status of protein kinases can provide the essential information for their activation. This review provides analytical techniques for analysis of phosphorylation status of protein kinases by Phos-tag SDS-PAGE. We believe that this review would help readers to study in kinomics research.
中文翻译：
通过 Phos-tag SDS-PAGE 分析蛋白激酶
蛋白激酶调节几乎所有的生物过程，包括细胞增殖、分化、凋亡和基因表达。由蛋白激酶的异常活性和表达引起的蛋白磷酸化失调导致各种疾病的发作，例如癌症和代谢综合征。大量蛋白激酶的活性受磷酸化调节。因此，分析蛋白激酶的磷酸化状态对于阐明生物现象和疾病的发病机制具有重要意义。为了研究蛋白质磷酸化，开发了磷酸结合标签分子“Phos-tag”。此外，还开发了各种使用 Phos-tag 的技术和工具，例如 Phos-tag SDS-PAGE，用于蛋白质磷酸化的分析和分析。这里，
意义
蛋白激酶在调节疾病的许多生物学过程和发病机制中起关键作用。确定蛋白激酶的磷酸化状态可以为其激活提供必要的信息。本综述提供了通过 Phos-tag SDS-PAGE 分析蛋白激酶磷酸化状态的分析技术。我们相信这篇综述将有助于读者进行动力学研究。
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