Abstract

Objective: Chlordimeform is a chemical pesticide that is highly carcinogenic and toxic. The purpose of this study was to establish an enzyme-linked immunosorbent assay (ELISA) method for the detection of chlordimeform in aquaculture and fish farming. Methods: Chlordimeform was coupled with bovine serum albumin (BSA) and ovalbumin (OVA) as carrier proteins. A chlordimeform-BSA conjugate was used as an immunogen, and chlordimeform-OVA was used as a coating antigen. Chlordimeform-BSA was used to immunize rabbits, and a polyclonal antibody was prepared. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was established to detect chlordimeform. Results: The working range of the established IC-ELISA method for chlordimeform detection was 1–20 ng/mL. The IC₅₀ was 3.126 ng/mL, and the lower limit of detection (LOD) of chlordimeform was 0.637 ng/mL. The recovery of chlordimeform from spiked water samples ranged from 81% to 107%. Conclusion: An anti-chlordimeform polyclonal antibody was successfully developed, and a novel IC-ELISA was established to detect chlordimeform in aquaculture.

摘要

目的：杀虫脒是一种化学农药，具有很强的致癌性和毒性。本研究的目的是建立一种酶联免疫吸附试验（ELISA）方法，用于检测水产养殖和鱼类养殖中的杀虫脒。方法：杀虫脒与牛血清白蛋白（BSA）和卵清蛋白（OVA）作为载体蛋白偶联。杀虫脒-BSA缀合物用作免疫原，杀虫脒-OVA用作包被抗原。用杀虫脒-BSA免疫家兔，制备多克隆抗体。建立了间接竞争酶联免疫吸附试验（IC-ELISA）检测杀虫脒。结果：建立的IC-ELISA检测杀虫脒的工作范围为1~20 ng/mL。IC ₅₀为3.126 ng/mL，杀虫脒的检测下限(LOD)为0.637 ng/mL。加标水样中杀虫脒的回收率为 81% 至 107%。结论：成功研制出抗敌草胺多克隆抗体，并建立了一种新型IC-ELISA检测水产养殖中杀虫脒的方法。