An electrochemical sensor for Vitamin B6 determination has been prepared by the electrochemical deposition of Pt nanospheres on graphene-β-CD coated Pencil Graphite Electrode (PGE). Cyclic voltammetric (CV) and electrochemical impedance spectroscopic (EIS) studies were employed to explore the electrochemical properties of the modified electrode. The physicochemical properties of the modified electrodes were characterized by X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and optical profilometric studies. The experimental conditions such as effect of scan rate, concentration and pH were optimized. The linear dynamic range for the determination of Vitamin B6 was found to be 5 nM to 205 nM. The low level of detection limit (1.2 nM) implies the high sensitivity of the process. The suggested method was effectively employed for the electrocatalytic evaluation of Vitamin B6 in different juice samples.

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