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Single-Electrode Electrochemical System for the Visual and High-Throughput Electrochemiluminescence Immunoassay
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-01-19 , DOI: 10.1021/acs.analchem.1c04709 Fangxin Du 1, 2 , Zhiyong Dong 1, 2 , Yiran Guan 1 , Abdallah M Zeid 1, 3 , Di Ma 4 , Jiachun Feng 4 , Di Yang 5 , Guobao Xu 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-01-19 , DOI: 10.1021/acs.analchem.1c04709 Fangxin Du 1, 2 , Zhiyong Dong 1, 2 , Yiran Guan 1 , Abdallah M Zeid 1, 3 , Di Ma 4 , Jiachun Feng 4 , Di Yang 5 , Guobao Xu 1, 2
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The electrochemiluminescence (ECL) immunoassay with its visual and high-throughput detection has received considerable attention in the past decade. However, the development of a facile and cost-effective ECL device is still a great challenge. Herein, a single-electrode electrochemical system (SEES) for the visual and high-throughput ECL immunoassay was developed. The SEES was designed by attaching a plastic sticker with multiple holes onto a single carbon ink screen-printed electrode based on a resistance-induced potential difference. Due to its excellent properties of adsorption and bioaffinity, the carbon ink screen-printed electrode is applied to immobilize antibodies. When cardiac troponin I (cTnI), a specific biomarker of acute myocardial infarction, is present, it will be captured by the immobilized cTnI antibodies on the electrode surface, inhibiting electron transfer, resulting in a decrease of the ECL intensity of the luminol–H2O2 system. Using a smartphone as the detector, cTnI could be determined, ranging from 1 to 1000 ng mL–1, with a detection limit of 0.94 ng mL–1. The SEES based on the carbon ink screen-printed electrode is characterized by its high simplicity, cost effectiveness, and user-friendliness compared with conventional three-electrode systems and bipolar electrochemical systems using electrode arrays and shows superior advantages over other immunoassay strategies, with the elimination of multistep assembling and labeling processes. What is more, the fabricated SEES holds great potential in the point-of-care testing due to its tiny size and the combination of a smartphone detector.
中文翻译：
用于视觉和高通量电化学发光免疫测定的单电极电化学系统
电化学发光 (ECL) 免疫测定法以其视觉和高通量检测在过去十年中受到了广泛关注。然而，开发一种简便且具有成本效益的 ECL 设备仍然是一个巨大的挑战。在此，开发了一种用于视觉和高通量 ECL 免疫测定的单电极电化学系统 (SEES)。SEES 是通过将带有多个孔的塑料贴纸贴在单个碳墨丝网印刷电极上而设计的，该电极基于电阻引起的电位差。由于其优异的吸附性和生物亲和性，碳墨丝网印刷电极被用于固定抗体。当心肌肌钙蛋白 I (cTnI) 是急性心肌梗死的特异性生物标志物存在时，它会被固定在电极表面的 cTnI 抗体捕获，2 O 2系统。使用智能手机作为检测器，可测定 cTnI，范围为 1 至 1000 ng mL –1，检测限为 0.94 ng mL –1。与使用电极阵列的传统三电极系统和双极电化学系统相比，基于碳油墨丝网印刷电极的 SEES 具有高度简单、成本效益和用户友好的特点，并且显示出优于其他免疫分析策略的优势，与消除多步组装和贴标签过程。更重要的是，制造的 SEES 由于其体积小和智能手机探测器的组合，在即时检测方面具有巨大潜力。
更新日期：2022-02-01
中文翻译：
用于视觉和高通量电化学发光免疫测定的单电极电化学系统
电化学发光 (ECL) 免疫测定法以其视觉和高通量检测在过去十年中受到了广泛关注。然而，开发一种简便且具有成本效益的 ECL 设备仍然是一个巨大的挑战。在此，开发了一种用于视觉和高通量 ECL 免疫测定的单电极电化学系统 (SEES)。SEES 是通过将带有多个孔的塑料贴纸贴在单个碳墨丝网印刷电极上而设计的，该电极基于电阻引起的电位差。由于其优异的吸附性和生物亲和性，碳墨丝网印刷电极被用于固定抗体。当心肌肌钙蛋白 I (cTnI) 是急性心肌梗死的特异性生物标志物存在时，它会被固定在电极表面的 cTnI 抗体捕获，2 O 2系统。使用智能手机作为检测器，可测定 cTnI，范围为 1 至 1000 ng mL –1，检测限为 0.94 ng mL –1。与使用电极阵列的传统三电极系统和双极电化学系统相比，基于碳油墨丝网印刷电极的 SEES 具有高度简单、成本效益和用户友好的特点，并且显示出优于其他免疫分析策略的优势，与消除多步组装和贴标签过程。更重要的是，制造的 SEES 由于其体积小和智能手机探测器的组合，在即时检测方面具有巨大潜力。
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