Per- and polyfluoroalkyl substances (PFAS) are ubiquitously detected in environmental and biological samples and cause adverse health effects. Studies have predominately focused on long-chain PFAS, with far fewer addressing short-chain alternatives. This study leveraged embryonic zebrafish to investigate developmental toxicity of a short-chain series: perfluorobutane sulfonate (PFBS), perfluoropentanoic acid (PFPeA), perfluorobutane sulfonamide (FBSA), and 4:2 fluorotelomer sulfonic acid (4:2 FTS). Following static exposures at 8 h postfertilization (hpf) to each chemical (1–100 μM), morphological and behavioral endpoints were assessed at 24 and 120 hpf. Only FBSA induced abnormal morphology, while exposure to all chemicals caused aberrant larval behavior. RNA sequencing at 48 hpf following 47 μM exposures revealed only FBSA significantly disrupted normal gene expression. Measured tissue concentrations were FBSA > PFBS > 4:2 FTS > PFPeA. This study demonstrates functional head groups impact bioactivity and bioconcentration.

全氟烷基物质和多氟烷基物质 (PFAS) 在环境和生物样品中无处不在，会对健康造成不利影响。研究主要集中在长链 PFAS 上，而针对短链替代品的研究则少得多。本研究利用胚胎斑马鱼来研究短链系列的发育毒性：全氟丁烷磺酸盐 (PFBS)、全氟戊酸 (PFP​​eA)、全氟丁磺酰胺 (FBSA) 和 4:2 氟调聚物磺酸 (4:2 FTS)。在受精后 8 小时 (hpf) 对每种化学物质 (1-100 μM) 进行静态暴露后，在 24 和 120 hpf 时评估形态和行为终点。只有 FBSA 诱导异常形态，而暴露于所有化学物质导致异常幼虫行为。47 μM 暴露后 48 hpf 的 RNA 测序显示只有 FBSA 显着破坏了正常基因表达。测量的组织浓度为 FBSA > PFBS > 4:2 FTS > PFPeA。该研究表明功能性头部基团影响生物活性和生物浓度。