Eupatin通过线粒体凋亡途径有效减轻顺铂诱导的听觉毛细胞死亡的新应用,Oxidative Medicine and Cellular Longevity

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Eupatin通过线粒体凋亡途径有效减轻顺铂诱导的听觉毛细胞死亡的新应用
Oxidative Medicine and Cellular Longevity Pub Date : 2022-01-17 , DOI: 10.1155/2022/1090034
Xiaochan Lu ₁ , Tingting Deng ₁ , Hongsong Dong ₁ , Jinghong Han ₁ , Yanping Yu ₁ , Deng Xiang ₁ , Guohui Nie ₁ , Bing Hu ₁

Affiliation

Eupatilin (5,7-dihydroxy-3 , 4 , 6-trimethoxyflavone) 是一种具有药理活性的黄酮，已从多种药用植物中分离出来，并具有多种药理特性。本研究在体外和体内评估了依帕替林对顺铂诱导的耳毒性的抗氧化和抗凋亡作用模型包括 HEI-OC1 细胞、耳蜗毛细胞和斑马鱼。采用 CCK8 测定和 Annexin V-FITC/PI 双染色，我们发现eupatin 显着减轻顺铂诱导的细胞凋亡并增加毛细胞活力。通过 CellROX green 和 MitoSOX Red 染色评估活性氧 (ROS) 的水平。结果表明eupatin具有抗氧化活性。MitoTracker Red 染色表明eupatin 显着降低了线粒体损伤。此外，我们证明了eupatin 保护毛细胞免受顺铂诱导的损伤。顺铂诱导的 HEI-OC1 细胞的机制研究表明，eupatin 促进 Bcl-2 表达，下调 Bax 表达，逆转 caspase-3 和 PARP 活性的增加，并降低磷酸化 p38 和 JNK 的表达。

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Novel Application of Eupatilin for Effectively Attenuating Cisplatin-Induced Auditory Hair Cell Death via Mitochondrial Apoptosis Pathway

Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a pharmacologically active flavone that has been isolated from a variety of medicinal plants and possesses a number of pharmacological properties. This study evaluates the antioxidant and antiapoptotic effects of eupatilin on cisplatin-induced ototoxicity using in vitro and in vivo models including HEI-OC1 cells, cochlear hair cells, and zebrafish. Employing a CCK8 assay and Annexin V-FITC/PI double staining, we found that eupatilin significantly alleviated cisplatin-induced apoptosis and increased hair cell viability. The level of reactive oxygen species (ROS) was evaluated by CellROX green and MitoSOX Red staining. The results showed that eupatilin possesses antioxidant activity. MitoTracker Red staining indicated that eupatilin remarkably decreased mitochondrial damage. Furthermore, we demonstrated that eupatilin protects hair cells from cisplatin-induced damage. Mechanistic studies in cisplatin-induced HEI-OC1 cells revealed that eupatilin promoted Bcl-2 expression, downregulated Bax expression, reversed the increase in caspase-3 and PARP activity, and reduced the expression of phosphorylated p38 and JNK. Our data suggest a novel role for eupatilin as a protective agent against ototoxic drug-induced hair cell apoptosis by inhibiting ROS generation and modulating mitochondrial-related apoptosis.

更新日期：2022-01-17

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