胶体铜纳米粒子对革兰氏阴性（大肠杆菌和变形杆菌）细菌的抗菌活性,Letters in Applied Microbiology

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胶体铜纳米粒子对革兰氏阴性（大肠杆菌和变形杆菌）细菌的抗菌活性
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2022-01-16 , DOI: 10.1111/lam.13655
P Sharma _{1,

2} , D Goyal ₁ , B Chudasama _{2,

3}

Affiliation

由于病原体的快速系统发育发展为多药耐药性（MDR），合成纳米颗粒的抗菌活性在过去几年中受到关注。铜纳米粒子 (CuNPs) 对替代致病革兰氏阴性菌大肠杆菌（MTCC no. 739）和普通变形杆菌的抗菌活性(MTCC no. 426) 在培养条件下进行评估。采用化学还原法合成了三组胶体CuNPs，每批产量分别为0·2、0·3和0·4 g。合成后的 CuNPs 具有相同的等离子体特性并具有相似的流体动力学粒径（11-14 nm）。通过最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 测试、细胞质渗漏和活性氧 (ROS) 测定来评估 CuNPs 的抗菌活性。MIC 和 MBC 测试显示剂量依赖性杀菌作用。E. coli的生长曲线显示出比P. vulgaris更快的生长抑制和更高的细胞质渗漏。这可能是因为大肠杆菌的膜通透性增加. CuNP-微生物相互作用诱导由 ROS 产生的氧化应激。细胞质成分的泄漏、膜通透性的丧失和 ROS 的产生是 CuNP 诱导的细菌细胞死亡的主要原因。合成后的 CuNPs 具有良好的抗菌活性，可能是新型抗菌剂的有希望的候选者。

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Antibacterial activity of colloidal copper nanoparticles against Gram-negative (Escherichia coli and Proteus vulgaris) bacteria

Antibacterial activities of as-synthesized nanoparticles have gained attention in past few years due to rapid phylogenesis of pathogens developing multi-drug resistance (MDR). Antibacterial activity of copper nanoparticles (CuNPs) on surrogate pathogenic Gram-negative bacteria Escherichia coli (MTCC no. 739) and Proteus vulgaris (MTCC no. 426) was evaluated under culture conditions. Three sets of colloidal CuNPs were synthesized by chemical reduction method with per batch yield of 0·2, 0·3 and 0·4 g. As-synthesized CuNPs possess identical plasmonic properties and have similar hydrodynamic particle sizes (11–14 nm). Antibacterial activities of CuNPs were evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests, cytoplasmic leakage and reactive oxygen species (ROS) assays. MIC and MBC tests revealed dose dependence bactericidal action. Growth curves of E. coli show faster growth inhibition along with higher cytoplasmic leakage than that of P. vulgaris. This might be because of increased membrane permeability of E. coli. CuNP–microorganism interaction induces oxidative stress generated by ROS. Leakage of cytoplasmic components, loss of membrane permeability and ROS generation are the primary causes of CuNP-induced bacterial cell death. As-synthesized CuNPs exhibiting promising antibacterial activities and could be a promising candidate for novel antibacterial agents.

更新日期：2022-01-16

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