13C Direct Detected NMR for Challenging Systems,Chemical Reviews

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13C Direct Detected NMR for Challenging Systems
Chemical Reviews ( IF 51.4 ) Pub Date : 2022-01-13 , DOI: 10.1021/acs.chemrev.1c00871
Isabella C Felli ₁ , Roberta Pierattelli ₁

Affiliation

Thanks to recent improvements in NMR spectrometer hardware and pulse sequence design, modern ¹³C NMR has become a useful tool for biomolecular applications. The complete assignment of a protein can be accomplished by using ¹³C detected multinuclear experiments and it can provide unique information relevant for the study of a variety of different biomolecules including paramagnetic proteins and intrinsically disordered proteins. A wide range of NMR observables can be measured, concurring to the structural and dynamic characterization of a protein in isolation, as part of a larger complex, or even inside a living cell. We present the different properties of ¹³C with respect to ¹H, which provide the rationale for the experiments developed and their application, the technical aspects that need to be faced, and the many experimental variants designed to address different cases. Application areas where these experiments successfully complement proton NMR are also described.

中文翻译：

适用于挑战性系统的 13C 直接检测 NMR

由于 NMR 波谱仪硬件和脉冲序列设计的最新改进，现代¹³ C NMR 已成为生物分子应用的有用工具。蛋白质的完整分配可以通过使用¹³ C 检测多核实验来完成，并且它可以提供与各种不同生物分子（包括顺磁性蛋白质和本质无序蛋白质）的研究相关的独特信息。可以测量各种 NMR 可观测值，与分离的蛋白质、作为较大复合物的一部分、甚至在活细胞内的蛋白质的结构和动态表征相一致。我们介绍了¹³ C 相对于¹ H 的不同性质，这为所开发的实验及其应用、需要面对的技术方面以及旨在解决不同情况的许多实验变体提供了基本原理。还描述了这些实验成功补充质子 NMR 的应用领域。

更新日期：2022-01-13

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