不同肠段附着的肠系膜脂肪组织特征及其在免疫调节中的作用,American Journal of Physiology-Gastrointestinal and Liver Physiology

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不同肠段附着的肠系膜脂肪组织特征及其在免疫调节中的作用
American Journal of Physiology-Gastrointestinal and Liver Physiology ( IF 3.9 ) Pub Date : 2022-01-05 , DOI: 10.1152/ajpgi.00256.2021
Haowei Zhang _{1,

2,

3} , Yujin Ding _{1,

2} , Qin Zeng _{1,

2} , Dandan Wang _{1,

2} , Ganglei Liu ₄ , Zain Hussain ₅ , Boen Xiao ₆ , Wei Liu ₆ , Tuo Deng _{1,

2,

7}

Affiliation

背景：肠系膜脂肪组织（MAT）在肠道生理生态系统中起着至关重要的作用。小肠和大肠具有明显的内在和鲜明的特征。然而，在小肠和大肠（SMAT 和 LMAT）附近是否存在任何肠系膜差异尚未得到正确表征。我们研究了这些差异的重要方面，例如 MAT 的形态、基因表达、细胞成分和免疫调节，以表征肠系膜差异。方法：利用小鼠SMAT和LMAT进行组织形态学比较。通过 RNA-seq 分析配对的肠系膜样本以阐明基因表达谱。构建MAT部分切除模型来说明MAT的免疫调节作用，并应用 16S-seq 检测随后对微生物群的影响。结果：我们的数据显示肠系膜的不同部分具有不同的形态结构。与 LMAT 相比，SMAT 不仅脂肪细胞更小，而且含有更多的脂肪相关淋巴团。小鼠中这两种 MAT 的基因表达谱也存在差异。B细胞标志物在SMAT中大量表达，而发育相关基因在LMAT中高度表达。与 SMAT 相比，LMAT 的脂肪源性干细胞表现出更高的脂肪形成潜力和更低的增殖率。此外，SMAT 和 LMAT 在免疫调节中发挥不同的作用，并随后影响微生物群成分。最后，我们的数据阐明了人类 SMAT 和 LMAT 之间所描述的差异。结论：

"点击查看英文标题和摘要"

The characteristics of mesenteric adipose tissue attached to different intestinal segments and their roles in immune regulation

Background: Mesenteric adipose tissue (MAT) plays a critical role in the intestinal physiological ecosystems. Small and large intestines have evidently intrinsic and distinct characteristics. However, whether there exist any mesenteric differences adjacent to the small and large intestines (SMAT and LMAT) has not been properly characterized. We studied the important facets of these differences, such as morphology, gene expression, cell components and immune regulation of MATs, to characterize the mesenteric differences. Methods: The SMAT and LMAT of mice were utilized for comparison of tissue morphology. Paired mesenteric samples were analyzed by RNA-seq to clarify gene expression profiles. MAT partial excision models were constructed to illustrate the immune regulation roles of MATs, and 16S-seq was applied to detect the subsequent effect on microbiota. Results: Our data show that different segments of mesenteries have different morphological structures. SMAT not only has smaller adipocytes but also contains more fat-associated lymphoid clusters than LMAT. The gene expression profile is also discrepant between these two MATs in mice. B-cell markers were abundantly expressed in SMAT, while development-related genes were highly expressed in LMAT. Adipose-derived stem cells of LMAT exhibited higher adipogenic potential and lower proliferation rates than those of SMAT. In addition, SMAT and LMAT play different roles in immune regulation and subsequently affect microbiota components. Finally, our data clarified the described differences between SMAT and LMAT in humans. Conclusions: There were significant differences in cell morphology, gene expression profiles, cell components, biological characteristics, and immune and microbiota regulation roles between regional MATs.

更新日期：2022-01-05

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