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Uncovering Nematicidal Natural Products from Xenorhabdus Bacteria
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-01-04 , DOI: 10.1021/acs.jafc.1c05454 Desalegne Abebew 1 , Fatemeh S Sayedain 2 , Edna Bode 2 , Helge B Bode 1, 2, 3
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-01-04 , DOI: 10.1021/acs.jafc.1c05454 Desalegne Abebew 1 , Fatemeh S Sayedain 2 , Edna Bode 2 , Helge B Bode 1, 2, 3
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Parasitic nematodes infect different species of animals and plants. Root-knot nematodes are members of the genus Meloidogyne, which is distributed worldwide and parasitizes numerous plants, including vegetables, fruits, and crops. To reduce the global burden of nematode infections, only a few chemical therapeutic classes are currently available. The majority of nematicides are prohibited due to their harmful effects on the environment and public health. This study was intended to identify new nematicidal natural products (NPs) from the bacterial genus Xenorhabdus, which exists in symbiosis with Steinernema nematodes. Cell-free culture supernatants of Xenorhabdus bacteria were used for nematicidal bioassay, and high mortality rates for Caenorhabditis elegans and Meloidogyne javanica were observed. Promoter exchange mutants of biosynthetic gene clusters encoding nonribosomal peptide synthetases (NRPS) or NRPS-polyketide synthase hybrids in Xenorhabdus bacteria carrying additionally a hfq deletion produce a single NP class, which have been tested for their bioactivity. Among the NPs tested, fabclavines, rhabdopeptides, and xenocoumacins were highly toxic to nematodes and resulted in mortalities of 95.3, 74.6, and 72.6% to C. elegans and 82.0, 90.0, and 85.3% to M. javanica, respectively. The findings of such nematicidal NPs can provide templates for uncovering effective and environmentally safe alternatives to commercially available nematicides.
中文翻译：
从 Xenorhabdus 细菌中发现杀线虫天然产物
寄生线虫感染不同种类的动植物。根结线虫是根结线虫属的成员，根结线虫分布于世界各地，寄生于许多植物，包括蔬菜、水果和农作物。为了减轻线虫感染的全球负担，目前只有少数几种化学疗法可供使用。大多数杀线虫剂因对环境和公众健康有害而被禁止使用。本研究旨在从与Steinernema线虫共生的细菌属Xenorhabdus中鉴定新的杀线虫天然产物 (NPs) 。异形杆菌的无细胞培养上清液细菌被用于杀线虫生物测定，并观察到秀丽隐杆线虫和爪哇根结线虫的高死亡率。编码非核糖体肽合成酶 (NRPS) 或 NRPS-聚酮化合物合酶杂合体的生物合成基因簇的启动子交换突变体在Xenorhabdus细菌中额外携带hfq缺失产生单个 NP 类，已对其生物活性进行了测试。在测试的 NPs 中，fabclavines、rhabdopeptides 和 xenocoumacins 对线虫具有高毒性，导致秀丽隐杆线虫的死亡率为 95.3%、74.6% 和 72.6%，而爪哇线虫的死亡率为82.0%、90.0% 和 85.3% 。， 分别。这种杀线虫 NPs 的发现可以提供模板，用于发现市售杀线虫剂的有效和环境安全的替代品。
更新日期：2022-01-19
中文翻译：
从 Xenorhabdus 细菌中发现杀线虫天然产物
寄生线虫感染不同种类的动植物。根结线虫是根结线虫属的成员，根结线虫分布于世界各地，寄生于许多植物，包括蔬菜、水果和农作物。为了减轻线虫感染的全球负担，目前只有少数几种化学疗法可供使用。大多数杀线虫剂因对环境和公众健康有害而被禁止使用。本研究旨在从与Steinernema线虫共生的细菌属Xenorhabdus中鉴定新的杀线虫天然产物 (NPs) 。异形杆菌的无细胞培养上清液细菌被用于杀线虫生物测定，并观察到秀丽隐杆线虫和爪哇根结线虫的高死亡率。编码非核糖体肽合成酶 (NRPS) 或 NRPS-聚酮化合物合酶杂合体的生物合成基因簇的启动子交换突变体在Xenorhabdus细菌中额外携带hfq缺失产生单个 NP 类，已对其生物活性进行了测试。在测试的 NPs 中，fabclavines、rhabdopeptides 和 xenocoumacins 对线虫具有高毒性，导致秀丽隐杆线虫的死亡率为 95.3%、74.6% 和 72.6%，而爪哇线虫的死亡率为82.0%、90.0% 和 85.3% 。， 分别。这种杀线虫 NPs 的发现可以提供模板，用于发现市售杀线虫剂的有效和环境安全的替代品。
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