柠檬草精油及其主要成分柠檬醛异构体调节 3T3-L1 细胞中的脂肪基因表达,Journal of Food Biochemistry

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柠檬草精油及其主要成分柠檬醛异构体调节 3T3-L1 细胞中的脂肪基因表达
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2022-01-03 , DOI: 10.1111/jfbc.14037
Steven Sprenger ₁ , Tibebe Woldemariam ₂ , Simeon Kotchoni ₂ , Hatem A Elshabrawy ₃ , Lakshmi Shankar Chaturvedi ₄

Affiliation

肥胖是糖尿病、高血压和冠状动脉疾病等疾病的易感因素。柠檬草精油 (LEO)，来自Cymbopogon flexuosus，具有许多治疗特性，包括在体内调节肥胖。本实验研究了 LEO 及其主要成分柠檬醛 (3,7-dimethyl-2,6-octadiinal)、柠檬醛二甲基乙缩醛 (1,1-dimethoxy-3,7-dimethylocta-2,6-diene) 和柠檬醛二乙缩醛 (1,1-diethoxy-3,7-dimethylocta-2,6-diene) 调节脂肪细胞中的脂肪生成和基因表达。从 ATCC 获得的小鼠 3T3-L1 前脂肪细胞诱导脂肪生成，并维持在富含小牛血清的 Dulbecco 改良 Eagle 培养基 (DMEM) 中。使用富含 10% 胎牛血清、地塞米松 0.25 µM、3-异丁基-甲基黄嘌呤 0.5 mM 和胰岛素 10 mg/ml 的 DMEM 进行分化 2 天，然后单独使用 10 mg/ml 胰岛素 5 天。样品以 2 的浓度进行实验处理。^-3. 在油红 O (ORO) 染色程序后对细胞内甘油三酯进行量化并获得显微照片。提取总核糖核酸并使用实时聚合酶链反应定量影响脂质代谢的基因的表达。ORO 染色程序和分光光度分析表明治疗后脂质积累减少。LEO及其主要成分显着抑制甾醇反应结合蛋白2、分化簇36、脂肪酸结合蛋白4和peripilin的表达。这些结果表明通过减少脂质摄取、增加脂肪分解、减少分化和下调脂质生物合成来调节脂质积累。这项研究表明 LEO 及其成分对脂肪细胞代谢产生影响，对于了解代谢疾病很重要。需要进一步研究以阐明每种机制对观察到的效果的贡献程度。

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Lemongrass essential oil and its major constituent citral isomers modulate adipogenic gene expression in 3T3-L1 cells

Obesity is a predisposing factor to diseases such as diabetes mellitus, hypertension, and coronary artery disease. Lemongrass essential oil (LEO), from Cymbopogon flexuosus, possesses numerous therapeutic properties including modulation of obesity in vivo. This experiment investigated the effect of LEO and its major components citral (3,7-dimethyl-2,6-octadienal), citral dimethyl acetal (1,1-dimethoxy-3,7-dimethylocta-2,6-diene), and citral diethyl acetal (1,1-diethoxy-3,7-dimethylocta-2,6-diene) in modulation of adipogenesis and genetic expression in adipocytes. Adipogenesis was induced from murine 3T3-L1 preadipocytes procured from ATCC and maintained in Dulbecco's modified Eagle's medium (DMEM) enriched with calf serum. Differentiation was conducted using DMEM enriched with 10% fetal bovine serum, Dexamethasone 0.25 µM, 3-isobutyl-methylxanthine 0.5 mM, and insulin 10 mg/ml for 2 days, followed by 5 days of insulin 10 mg/ml alone. Samples were subjected to experimental treatments at a concentration of 2.5 × 10⁻³. Intracellular triglycerides were quantified and photomicrographs were obtained following Oil red O (ORO) staining procedure. Total ribonucleic acid was extracted and expression of genes effecting in lipid metabolism were quantitated using real-time polymerase chain reaction. ORO staining procedure and spectrophotometric analysis demonstrated decreased lipid accumulation following treatments. LEO and its major constituents significantly inhibited expression of sterol response binding protein 2, cluster of differentiation 36, fatty acid binding protein 4, and peripilin. These results indicate modulation of lipid accumulation through decreased lipid uptake, increased lipolysis, decreased differentiation, and downregulated lipid biosynthesis. This investigation suggests that LEO and its constituents exert effects on adipocyte metabolism and are important for understanding metabolic disease. Further investigation is required to elucidate the degree that each mechanism implicated contributes to the observed effect.

更新日期：2022-02-10

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