转阿魏酸在体外通过激活 AMPK 信号通路减轻高血糖诱导的氧化应激并调节糖代谢,Journal of Food Biochemistry

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转阿魏酸在体外通过激活 AMPK 信号通路减轻高血糖诱导的氧化应激并调节糖代谢
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2022-01-03 , DOI: 10.1111/jfbc.14038
Sangeetha S B Singh _{1,

2} , K Neelakanteshwar Patil _{1,

2}

Affiliation

腺苷一磷酸活化蛋白激酶 (AMPK) 是一种有效的代谢调节剂，也是抗糖尿病激活剂的一个有吸引力的靶标。在这里，我们首次报道，转阿魏酸 (TFA) 是羟基肉桂酸衍生物的有效膳食生物活性分子，用于激活 AMPK，磷酸化增加最大 (2.71/2.67 ± 0.10; p < .001 vs. 高血糖 [HG] 对照）在高血糖诱导的人类肝细胞 (HepG2) 和大鼠骨骼肌细胞 (L6) 中，其中 HG 抑制 AMPK 通路。还观察到 TFA 以剂量和时间依赖性方式增加 AMPK 的活化，并增加乙酰辅酶 A 羧化酶 (ACC) 的磷酸化，表明它可能促进脂肪酸氧化；然而，用化合物 C 预处理逆转了这种效果。此外，TFA 降低了由高血糖引起的细胞内活性氧 (ROS) 和一氧化氮 (NO) 的水平，随后增加了谷胱甘肽的水平。有趣的是，TFA 还上调了葡萄糖转运蛋白 GLUT2 和 GLUT4，并通过降低 HG 条件下测试细胞的磷酸化水平来抑制 c-Jun N-末端蛋白激酶 (JNK1/2)。

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trans-ferulic acid attenuates hyperglycemia-induced oxidative stress and modulates glucose metabolism by activating AMPK signaling pathway in vitro

Adenosine monophosphate-activated protein kinase (AMPK) is a potent metabolic regulator and an attractive target for antidiabetic activators. Here we report for the first that, trans-ferulic acid (TFA) is a potent dietary bioactive molecule of hydroxycinnamic acid derivative for the activation of AMPK with a maximum increase in phosphorylation (2.71/2.67 ± 0.10; p < .001 vs. high glucose [HG] control) in hyperglycemia-induced human liver cells (HepG2) and rat skeletal muscle cells (L6), where HG suppresses the AMPK pathway. It was also observed that TFA increased activation of AMPK in a dose- and time-dependent manner and also increased the phosphorylation of acetyl-CoA carboxylase (ACC), suggesting that it may promotes fatty acid oxidation; however, pretreatment with compound C reversed the effect. In addition, TFA reduced the level of intracellular reactive oxygen species (ROS) and nitric oxide (NO) induced by hyperglycemia and subsequently increased the level of glutathione. Interestingly, TFA also upregulated the glucose transporters, GLUT2 and GLUT4, and inhibited c-Jun N-terminal protein kinase (JNK1/2) by decreasing the phosphorylation level in tested cells under HG condition. Our studies provide critical insights into the mechanism of action of TFA as a potential natural activator of AMPK under hyperglycemia.

更新日期：2022-02-10

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