<br>酵母核孔复合体的全面结构和功能适应,Cell

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酵母核孔复合体的全面结构和功能适应
Cell ( IF 45.5 ) Pub Date : 2022-01-03 , DOI: 10.1016/j.cell.2021.12.015
Christopher W Akey ₁ , Digvijay Singh ₂ , Christna Ouch ₃ , Ignacia Echeverria ₄ , Ilona Nudelman ₅ , Joseph M Varberg ₆ , Zulin Yu ₆ , Fei Fang ₇ , Yi Shi ₇ , Junjie Wang ₈ , Daniel Salzberg ₉ , Kangkang Song ₁₀ , Chen Xu ₁₀ , James C Gumbart ₁₁ , Sergey Suslov ₂ , Jay Unruh ₆ , Sue L Jaspersen ₁₂ , Brian T Chait ₈ , Andrej Sali ₁₃ , Javier Fernandez-Martinez ₅ , Steven J Ludtke ₁₄ , Elizabeth Villa ₁₅ , Michael P Rout ₅

Affiliation

Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA.
Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA.
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA; Department of Cellular and Molecular Pharmacology, San Francisco, San Francisco, CA 94158, USA.
Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA.
Stowers Institute for Medical Research, Kansas City, MO, USA.
Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, USA.
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA.
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA.
School of Physics, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Stowers Institute for Medical Research, Kansas City, MO, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA; Quantitative Biosciences Institute, University of California San Francisco, San Francisco, CA 94158, USA; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030, USA.
Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093, USA.

核孔复合物（NPC）介导大分子的核细胞质运输。在这里，我们提供了分离的酵母 NPC 的结构，其中内环通过冷冻电镜以亚纳米分辨率解析，以展示柔性连接器如何将不同的结构和功能层连接在一起。这些连接器可能是具有开放有丝分裂的细胞中磷酸化和受调节分解的靶标。此外，一些核孔蛋白对和转运因子具有相似的相互作用基序，这表明组装和转运之间存在进化和机制联系。我们提供了三种主要 NPC 变体的证据，这可能预示着核外围的功能专门化。冷冻电子断层扫描扩展了这些研究，提供了具有径向扩张内环的原位NPC 模型。我们的综合模型揭示了核篮和中央转运蛋白的特征，表明了腔 Pom152 环在限制扩张中的作用，并强调了运输可能所需的结构可塑性。

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更新日期：2022-01-20

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