Here, we describe the synthesis and properties of BMeS-p-A-NHS as a sulfonylaniline-based fluorescent labeling reagent. Introduction of a carboxyl group into the amino group of the sulfonylaniline skeleton was achieved by the ring-opening reaction of β-propiolactone. This small labeling reagent was water-soluble, photostable, solid-state emissive, and suitable for excitation with a versatile 405-nm laser diode, emitting green fluorescence with a large Stokes shift at 527 nm. The succinimidyl ester reactive group facilitated conjugation with substrates containing amino groups.

中文翻译：

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BMeS-pA 琥珀酰亚胺酯作为磺酰苯胺染料标记试剂

在这里，我们描述了 BMeS- p -A-NHS 作为磺酰苯胺基荧光标记试剂的合成和性质。通过β-丙内酯的开环反应将羧基引入磺酰苯胺骨架的氨基中。这种小型标记试剂是水溶性的、光稳定的、固态发射的，适合用多功能 405 nm 激光二极管激发，在 527 nm 处发出具有大斯托克斯位移的绿色荧光。琥珀酰亚胺酯反应性基团促进与含有氨基的底物结合。