Objective. We analyzed the efficacy and pharmacological mechanisms of action of Zhen Ren Yang Zang decoction (ZRYZD) on ulcerative colitis (UC) using meta-analysis and network pharmacology. Methods. The major databases were searched for randomized controlled trials of ZRYZD for the treatment of UC. Meta-analysis of the efficacy of ZRYZD on UC was conducted using RevMan software. Active compounds and target genes were acquired using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. UC-related genes were searched using the GeneCards database. Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using RGUI. A compound-target network was constructed using Cytoscape software, and a protein-protein interaction network was constructed using the STRING database. Molecular docking simulations of the macromolecular protein targets and their corresponding ligand compounds were performed using the AutoDock tool and AutoDock Vina software. Results. Meta-analysis revealed that the total effective rate and recovery rate of clinical efficacy were significantly higher in the experimental group than those of the control group. The screening identified 169 active compounds and 277 active target genes for ZRYZD. The 277 active target genes were compared with the 4,798 UC-related genes. This identified 187 active target genes of ZRYZD for UC that correlated with 138 active compounds. GO functional enrichment and KEGG pathway enrichment analyses were performed, and compound-target and protein-protein interaction networks were constructed. The key compounds and key target proteins were then selected. Finally, target protein binding with the corresponding compound was analyzed using molecular docking. Conclusion. Our findings demonstrate the effectiveness and safety of ZRYZD for the treatment of UC and provide insight into the underlying pharmacological mechanisms of action. Furthermore, key compounds were identified, laying the foundation for future studies on ZRYZD for the treatment of UC.

中文翻译：

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Meta分析和网络药理学分析真人养脏汤治疗溃疡性结肠炎的疗效及药理机制


客观的。我们利用荟萃分析和网络药理学分析了真人养脏汤（ZRYZD）对溃疡性结肠炎（UC）的功效和药理学作用机制。方法。在主要数据库中检索了 ZRYZD 治疗 UC 的随机对照试验。使用 RevMan 软件对 ZRYZD 对 UC 的疗效进行荟萃分析。使用中药系统药理学数据库和分析平台获取活性化合物和靶基因。使用 GeneCards 数据库搜索 UC 相关基因。使用 RGUI 进行基因本体 (GO) 功能富集和京都基因和基因组百科全书 (KEGG) 通路富集分析。使用Cytoscape软件构建化合物-靶标网络，并使用STRING数据库构建蛋白质-蛋白质相互作用网络。使用AutoDock工具和AutoDock Vina软件对大分子蛋白质靶标及其相应的配体化合物进行分子对接模拟。结果。 Meta分析显示，实验组的总有效率和临床疗效恢复率均显着高于对照组。筛选鉴定出 ZRYZD 的 169 种活性化合物和 277 种活性靶基因。将 277 个活性靶基因与 4,798 个 UC 相关基因进行比较。这鉴定了 187 个 ZRYZD UC 活性靶基因，与 138 种活性化合物相关。进行GO功能富集和KEGG通路富集分析，构建化合物-靶点和蛋白质-蛋白质相互作用网络。然后选择关键化合物和关键目标蛋白。 最后，使用分子对接分析目标蛋白与相应化合物的结合。结论。我们的研究结果证明了 ZRYZD 治疗 UC 的有效性和安全性，并提供了对潜在药理学作用机制的深入了解。此外，还确定了关键化合物，为未来 ZRYZD 治疗 UC 的研究奠定了基础。