延迟的胼胝质降解恢复了拟南芥中多个 P/TGMS 系的生育力,Journal of Integrative Plant Biology

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延迟的胼胝质降解恢复了拟南芥中多个 P/TGMS 系的生育力
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2021-12-27 , DOI: 10.1111/jipb.13205
Kai-Qi Wang ₁ , Ya-Hui Yu ₁ , Xin-Lei Jia ₂ , Si-Da Zhou ₂ , Fang Zhang ₂ , Xin Zhao ₂ , Ming-Yue Zhai ₂ , Yi Gong ₂ , Jie-Yang Lu ₂ , Yuyi Guo ₂ , Nai-Ying Yang ₂ , Shui Wang ₂ , Xiao-Feng Xu ₂ , Zhong-Nan Yang ₂

Affiliation

光周期/温度敏感的基因雄性不育（P/TGMS）被广泛应用于提高作物产量。以前使用可逆雄性不育( rvms ) 突变体的研究表明，缓慢发育是拟南芥P/TGMS 系恢复生育力的一般机制。在这项工作中，我们分离了rvms-2 ( res3 ) 的恢复者，因为 rvms- 2的雄性不育被res3拯救了。表型分析和分子克隆表明，在res3中UPEX1 l的点突变导致从绒毡层到小室的callase A6分泌延迟和四分体胼胝质壁降解。电泳迁移率变动分析和染色质免疫沉淀分析表明，绒毡层转录因子 ABORTED MICROSPORES 直接调节UPEX1的表达，揭示了绒毡层分泌功能的途径。转基因系中胼胝质壁的早期降解消除了res3的育性恢复作用。res3也恢复了多个具有花粉壁缺陷的已知 P/TGMS 品系的生育力. 我们提出残留的胼胝质壁可以通过为花粉形成提供保护来广泛地补偿 P/TGMS 系的花粉壁缺陷。提出了一种细胞机制来解释缓慢的发育如何恢复拟南芥P/TGMS 系的生育力。

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Delayed callose degradation restores the fertility of multiple P/TGMS lines in Arabidopsis

Photoperiod/temperature-sensitive genic male sterility (P/TGMS) is widely applied for improving crop production. Previous investigations using the reversible male sterile (rvms) mutant showed that slow development is a general mechanism for restoring fertility to P/TGMS lines in Arabidopsis. In this work, we isolated a restorer of rvms–2 (res3), as the male sterility of rvms–2 was rescued by res3. Phenotype analysis and molecular cloning show that a point mutation in UPEX1 l in res3 leads to delayed secretion of callase A6 from the tapetum to the locule and tetrad callose wall degradation. Electrophoretic mobility shift assay and chromatin immunoprecipitation analysis demonstrated that the tapetal transcription factor ABORTED MICROSPORES directly regulates UPEX1 expression, revealing a pathway for tapetum secretory function. Early degradation of the callose wall in the transgenic line eliminated the fertility restoration effect of res3. The fertility of multiple known P/TGMS lines with pollen wall defects was also restored by res3. We propose that the remnant callose wall may broadly compensate for the pollen wall defects of P/TGMS lines by providing protection for pollen formation. A cellular mechanism is proposed to explain how slow development restores the fertility of P/TGMS lines in Arabidopsis.

更新日期：2021-12-27

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