多组学分析揭示了马铃薯块茎类固醇糖生物碱代谢的核心调控机制,Journal of Agricultural and Food Chemistry

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多组学分析揭示了马铃薯块茎类固醇糖生物碱代谢的核心调控机制
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-12-24 , DOI: 10.1021/acs.jafc.1c06867
Dan-Dan Shen ₁ , Ying-Peng Hua ₁ , Jin-Yong Huang ₁ , Shu-Ting Yu ₂ , Tai-Bo Wu ₂ , Yannning Zhang ₂ , Huan-Li Chen ₃ , Cai-Peng Yue ₁

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发芽的马铃薯块茎中的甾体糖苷生物碱 (SGA) 是有毒的；然而，人们对 SGA 代谢的潜在机制知之甚少。因此，在本研究中进行了整合的转录组、代谢组和激素分析，以识别和表征与配糖生物碱调节相关的关键调节基因、代谢物和植物激素。基于发芽和休眠马铃薯块茎芽眼的转录组测序，共鉴定出6260个差异表达基因，主要负责植物激素信号转导、碳水化合物代谢和次生代谢物生物合成。两个 TCP14 基因被确定为可能调节 SGA 合成的核心转录因子。代谢物分析表明，检测到 149 种显着不同的代谢物，它们富含次生代谢物的代谢和生物合成途径。在这些途径中，α-茄碱含量增加，与配糖生物碱生物合成相关的基因表达上调。赤霉素和茉莉酸含量升高，而脱落酸含量降低。该研究为研究SGAs的生物合成及调控奠定了基础，为马铃薯块茎的生产和消费提供了参考。

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Multiomic Analysis Reveals Core Regulatory Mechanisms underlying Steroidal Glycoalkaloid Metabolism in Potato Tubers

Steroidal glycoalkaloids (SGAs) present in germinated potato tubers are toxic; however, the mechanisms underlying SGA metabolism are poorly understood. Therefore, integrated transcriptome, metabolome, and hormone analyses were performed in this study to identify and characterize the key regulatory genes, metabolites, and phytohormones related to glycoalkaloid regulation. Based on transcriptome sequencing of bud eyes of germinated and dormant potato tubers, a total of 6260 differentially expressed genes were identified, which were mainly responsible for phytohormone signal transduction, carbohydrate metabolism, and secondary metabolite biosynthesis. Two TCP14 genes were identified as the core transcription factors that potentially regulate SGA synthesis. Metabolite analysis indicated that 149 significantly different metabolites were detected, and they were enriched in metabolic and biosynthetic pathways of secondary metabolites. In these pathways, the α-solanine content was increased and the expression of genes related to glycoalkaloid biosynthesis was upregulated. Levels of gibberellin and jasmonic acid were increased, whereas that of abscisic acid was decreased. This study lays a foundation for investigating the biosynthesis and regulation of SGAs and provides the reference for the production and consumption of potato tubers.

更新日期：2022-01-12

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