Enhanced Photostability of Genetically Encodable Fluoromodules Based on Fluorogenic Cyanine Dyes and a Promiscuous Protein Partner,Journal of the American Chemical Society

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Enhanced Photostability of Genetically Encodable Fluoromodules Based on Fluorogenic Cyanine Dyes and a Promiscuous Protein Partner
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2009-09-16 , DOI: 10.1021/ja9016864
Nathaniel I Shank ₁ , Kimberly J Zanotti , Frederick Lanni , Peter B Berget , Bruce A Armitage

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Fluoromodules are discrete complexes of biomolecules and fluorogenic dyes. Binding of the dyes to their cognate biomolecule partners results in enhanced dye fluorescence. We exploited a previously reported promiscuous binding interaction between a single-chain, variable fragment antibody protein and a family of cyanine dyes to create new protein-dye fluoromodules that exhibit enhanced photostability while retaining high affinity protein-dye binding. Modifications to the dye structure included electron-withdrawing groups that provide resistance to photo-oxidative damage. Low nanomolar equilibrium dissociation constants were found for the new dyes. Fluorescence microscopy illustrates how yeast can be surface-labeled with three different colors based on a single protein and appropriately chosen dyes.

中文翻译：

基于荧光花青染料和混杂蛋白质伙伴的遗传可编码荧光模块的光稳定性增强

荧光模块是生物分子和荧光染料的离散复合物。染料与其同源生物分子伴侣的结合导致染料荧光增强。我们利用先前报道的单链可变片段抗体蛋白与花青染料家族之间的混杂结合相互作用来创建新的蛋白质染料氟模块，该模块表现出增强的光稳定性，同时保留高亲和力蛋白质染料结合。对染料结构的修饰包括吸电子基团，可提供对光氧化损伤的抵抗力。发现新染料具有低纳摩尔平衡解离常数。Fluorescence microscopy illustrates how yeast can be surface-labeled with three different colors based on a single protein and appropriately chosen dyes.

更新日期：2009-09-16

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