A General Protein Unfolding-Chemical Coupling Strategy for Pure Protein Hydrogels with Mechanically Strong and Multifunctional Properties,Advanced Science

当前位置： X-MOL 学术 › Adv. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A General Protein Unfolding-Chemical Coupling Strategy for Pure Protein Hydrogels with Mechanically Strong and Multifunctional Properties
Advanced Science ( IF 14.3 ) Pub Date : 2021-12-22 , DOI: 10.1002/advs.202102557
Ziqing Tang ₁ , Huacheng He ₂ , Lin Zhu ₃ , Zhuangzhuang Liu ₄ , Jia Yang ₄ , Gang Qin ₄ , Jiang Wu ₅ , Yijing Tang ₆ , Dong Zhang ₆ , Qiang Chen _{1,

3,

7} , Jie Zheng ₆

Affiliation

Protein-based hydrogels have attracted great attention due to their excellent biocompatible properties, but often suffer from weak mechanical strength. Conventional strengthening strategies for protein-based hydrogels are to introduce nanoparticles or synthetic polymers for improving their mechanical strength, but often compromise their biocompatibility. Here, a new, general, protein unfolding-chemical coupling (PNC) strategy is developed to fabricate pure protein hydrogels without any additives to achieve both high mechanical strength and excellent cell biocompatibility. This PNC strategy combines thermal-induced protein unfolding/gelation to form a physically-crosslinked network and a -NH2/-COOH coupling reaction to generate a chemicallycrosslinked network. Using bovine serum albumin (BSA) as a globular protein, PNC-BSA hydrogels show macroscopic transparency, high stability, high mechanical properties (compressive/tensile strength of 115/0.43 MPa), fast stiffness/toughness recovery of 85%/91% at room temperature, good fatigue resistance, and low cell cytotoxicity and red blood cell hemolysis. More importantly, the PNC strategy can be not only generally applied to silk fibroin, ovalbumin, and milk albumin protein to form different, high strength protein hydrogels, but also modified with PEDOT/PSS nanoparticles as strain sensors and fluorescent fillers as color sensors. This work demonstrates a new, universal, PNC method to prepare high strength, multi-functional, pure protein hydrogels beyond a few available today.

中文翻译：

具有机械强度和多功能特性的纯蛋白质水凝胶的通用蛋白质解折叠化学偶联策略

蛋白质水凝胶因其优异的生物相容性而受到广泛关注，但往往机械强度较弱。基于蛋白质的水凝胶的传统强化策略是引入纳米颗粒或合成聚合物以提高其机械强度，但往往会损害其生物相容性。在这里，开发了一种新的、通用的蛋白质去折叠化学偶联（PNC）策略来制造不含任何添加剂的纯蛋白质水凝胶，以实现高机械强度和优异的细胞生物相容性。这种 PNC 策略结合了热诱导蛋白质解折叠/凝胶化以形成物理交联网络和 -NH2/-COOH 偶联反应以生成化学交联网络。 PNC-BSA水凝胶采用牛血清白蛋白（BSA）作为球状蛋白，具有宏观透明度、高稳定性、高机械性能（压缩/拉伸强度为115/0.43 MPa）、快速刚度/韧性恢复85%/91%常温下，耐疲劳性好，细胞毒性和红细胞溶血性低。更重要的是，PNC策略不仅可以普遍应用于丝素蛋白、卵清蛋白和乳白蛋白形成不同的高强度蛋白质水凝胶，还可以用PEDOT/PSS纳米颗粒作为应变传感器和荧光填料作为颜色传感器进行修饰。这项工作展示了一种新的、通用的 PNC 方法来制备高强度、多功能、纯蛋白质水凝胶，超越了目前可用的几种方法。

更新日期：2021-12-22

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南