Based on coumarin and benzopyran derivatives, a dual-wavelength excitation ratiometric fluorescent probe, HABA, was prepared to detect H₂S. The HABA probe showed good selectivity and anti-interference abilities during H₂S detection. Fluorescence co-localization experiments showed that HABA had excellent localization abilities toward mitochondria and lysosomes. More importantly, HABA can not only detect exogenous H₂S, but it can also detect endogenous H₂S, indicating that HABA has high application potential and value in the biological field.

中文翻译：

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用于检测细胞内 H2S 的线粒体和溶酶体靶向比率探针

基于香豆素和苯并吡喃衍生物，制备了双波长激发比率荧光探针HABA，用于检测H _{2 S。HABA探针在H 2} S检测中表现出良好的选择性和抗干扰能力。荧光共定位实验表明HABA对线粒体和溶酶体具有优异的定位能力。更重要的是，HABA不仅可以检测外源性H ₂ S，还可以检测内源性H ₂ S，说明HABA在生物领域具有很高的应用潜力和价值。