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Monolithic Gold Nanoparticles/Thiol-β-cyclodextrin-Functionalized TiO2 Nanowires for Enhanced SALDI MS Detection and Imaging of Natural Products
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-12-21 , DOI: 10.1021/acs.analchem.1c03764
Xian-Na Wang 1 , Bin Li 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-12-21 , DOI: 10.1021/acs.analchem.1c03764
Xian-Na Wang 1 , Bin Li 1
Affiliation
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Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) has been successfully applied in the analysis of various small molecules. In this work, gold nanoparticles/thiol-β-cyclodextrin-functionalized TiO2 nanowires (AuNPs/SH-β-CD-TiO2 NWs) were prepared to enhance the performance of SALDI MS and mass spectrometry imaging (MSI). A monolithic TiO2 film was first grown on an indium tin oxide (ITO) glass slide via a modified sol–gel method and treated in an alkaline environment to form nanowires. TiO2 NWs were chemically modified by SH-β-CD for immobilizing AuNPs densely and strongly. Compared with the conventional organic matrix 2,5-dihydroxybenzoic acid (DHB), the prepared AuNPs/SH-β-CD-TiO2 NWs showed superior performances on detection sensitivity, repeatability, and analyte coverage. Analytes typically detectable with negative-ion matrix-assisted laser desorption/ionization (MALDI) MS could also be observed using AuNPs/SH-β-CD-TiO2 NWs in the positive ion mode. Its successful usage efficiently enhanced the SALDI MS detection of various small molecules such as carbohydrates, fatty acids, and bile acids in the positive ion mode. The developed SALDI substrate was further used to characterize and discriminate the natural and in vitro cultured Calculus Bovis, as well as natural and artificial Moschus. Furthermore, the spatial distribution of several natural products in spearmint leaves and potato tubers was explored by tissue imprinting and deposition on the AuNPs/SH-β-CD-TiO2 NW surface for SALDI MSI in dual-polarity mode, respectively. The wide application and satisfied detection sensitivity make AuNPs/SH-β-CD-TiO2 NWs ideal for SALDI MS and MSI of various natural products.
中文翻译:
用于增强 SALDI MS 检测和天然产物成像的单片金纳米颗粒/硫醇-β-环糊精功能化 TiO2 纳米线
表面辅助激光解吸/电离质谱(SALDI MS)已成功应用于各种小分子的分析。在这项工作中,制备了金纳米颗粒/硫醇-β-环糊精功能化的 TiO 2纳米线 (AuNPs/SH-β-CD-TiO 2 NWs),以提高 SALDI MS 和质谱成像 (MSI) 的性能。首先通过改进的溶胶-凝胶法在氧化铟锡 (ITO) 载玻片上生长单片 TiO 2薄膜,并在碱性环境中处理形成纳米线。TiO 2 NWs 通过 SH-β-CD 进行化学修饰,以致密且牢固地固定 AuNPs。与常规有机基质2,5-二羟基苯甲酸(DHB)相比,制备的AuNPs/SH-β-CD-TiO 2NW 在检测灵敏度、重复性和分析物覆盖率方面表现出卓越的性能。使用负离子基质辅助激光解吸/电离 (MALDI) MS 通常可检测的分析物也可以在正离子模式下使用 AuNPs/SH-β-CD-TiO 2纳米线进行观察。它的成功使用有效地增强了 SALDI MS 在正离子模式下对碳水化合物、脂肪酸和胆汁酸等各种小分子的检测。开发的 SALDI 底物进一步用于表征和区分天然和体外培养的牛黄,以及天然和人造麝香。此外,通过AuNPs/SH-β-CD-TiO 2上的组织印迹和沉积,探索了几种天然产物在留兰香叶和马铃薯块茎中的空间分布。分别为双极性模式下 SALDI MSI 的 NW 表面。广泛的应用和令人满意的检测灵敏度使AuNPs/SH-β-CD-TiO 2纳米线成为各种天然产物的SALDI MS和MSI的理想选择。
更新日期:2022-01-18
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
用于增强 SALDI MS 检测和天然产物成像的单片金纳米颗粒/硫醇-β-环糊精功能化 TiO2 纳米线
表面辅助激光解吸/电离质谱(SALDI MS)已成功应用于各种小分子的分析。在这项工作中,制备了金纳米颗粒/硫醇-β-环糊精功能化的 TiO 2纳米线 (AuNPs/SH-β-CD-TiO 2 NWs),以提高 SALDI MS 和质谱成像 (MSI) 的性能。首先通过改进的溶胶-凝胶法在氧化铟锡 (ITO) 载玻片上生长单片 TiO 2薄膜,并在碱性环境中处理形成纳米线。TiO 2 NWs 通过 SH-β-CD 进行化学修饰,以致密且牢固地固定 AuNPs。与常规有机基质2,5-二羟基苯甲酸(DHB)相比,制备的AuNPs/SH-β-CD-TiO 2NW 在检测灵敏度、重复性和分析物覆盖率方面表现出卓越的性能。使用负离子基质辅助激光解吸/电离 (MALDI) MS 通常可检测的分析物也可以在正离子模式下使用 AuNPs/SH-β-CD-TiO 2纳米线进行观察。它的成功使用有效地增强了 SALDI MS 在正离子模式下对碳水化合物、脂肪酸和胆汁酸等各种小分子的检测。开发的 SALDI 底物进一步用于表征和区分天然和体外培养的牛黄,以及天然和人造麝香。此外,通过AuNPs/SH-β-CD-TiO 2上的组织印迹和沉积,探索了几种天然产物在留兰香叶和马铃薯块茎中的空间分布。分别为双极性模式下 SALDI MSI 的 NW 表面。广泛的应用和令人满意的检测灵敏度使AuNPs/SH-β-CD-TiO 2纳米线成为各种天然产物的SALDI MS和MSI的理想选择。