用于视网膜母细胞瘤化学/光疗的多功能聚合物纳米颗粒,ACS Biomaterials Science & Engineering

当前位置： X-MOL 学术 › ACS Biomater. Sci. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于视网膜母细胞瘤化学/光疗的多功能聚合物纳米颗粒
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-12-22 , DOI: 10.1021/acsbiomaterials.1c01234
Sushma Venkata Mudigunda ₁ , Deepak B Pemmaraju ₂ , Shivangi Paradkar ₁ , Eswara Rao Puppala ₂ , Basveshwar Gawali ₂ , Suryanarayana Murty Upadhyayula ₂ , Naidu Vegi Gangamodi ₂ , Aravind Kumar Rengan ₁

Affiliation

视网膜母细胞瘤 (Rb) 是发生在儿童中最严重和最严重的眼内恶性肿瘤。尽管医学策略取得了进步，但由于早期检测和控制失败，视网膜母细胞瘤的临床管理仍然具有挑战性。早期 Rb 肿瘤不占据主要视野，因此使用生物相容性材料的化学/光热疗法 (PTT) 可能是一种实用的方法。在这里，我们报告了作为化学/光热剂的多功能聚合物纳米粒子 (PNP)，其中包含 FDA 批准的抗癌药物 Palbociclib (PCB) 和近红外染料 IR820 (IR)。评估了这些 PCB/IR PNP 在视网膜母细胞瘤细胞系中的组合效应。此外，在小鼠模型中研究了 PNP 的体内光声成像功效和急性毒性特征。结果表明，PCB/IR PNPs 在 Y79 细胞系中表现出显着的细胞毒性作用 (86.5 ± 2.3%)，而不是暴露于 NIR 光下的相应对照组。定性和定量分析表明，具有 NIR 光诱导的 PCB/IR PNPs 导致 DNA 损伤，然后是细胞凋亡。PCB/IR PNP 在体内测试时显示出最佳的光声信号。因此，PCB 和 PTT 的组合可以成为视网膜母细胞瘤治疗的一种转化方式。

"点击查看英文标题和摘要"

Multifunctional Polymeric Nanoparticles for Chemo/Phototheranostics of Retinoblastoma

Retinoblastoma (Rb) is the most critical and severe intraocular malignancy occurring in children. The clinical management of retinoblastoma is still challenging due to failure in early detection and control despite the advancements in medical strategies. Early-stage Rb tumors do not occupy major visual fields, so chemo/photothermal therapy (PTT) with biocompatible materials can be a practical approach. Herein, we report multifunctional polymeric nanoparticles (PNPs) entrapped with an FDA-approved anticancer drug, Palbociclib (PCB), and a near-infrared dye, IR820 (IR), as chemo/photothermal agents. These PCB/IR PNPs were evaluated for the combinational effect in the retinoblastoma cell line. Further, the in vivo photoacoustic imaging efficacy and acute toxicity profile of the PNPs were studied in a mice model. The results indicated that the PCB/IR PNPs exhibited a significant cytotoxic effect (86.5 ± 2.3%) in Y79 cell lines than the respective control groups upon exposure to NIR light. Qualitative and quantitative analyses indicated that PCB/IR PNPs with NIR light induction resulted in DNA damage followed by apoptosis. PCB/IR PNPs, when tested in vivo, showed optimal photoacoustic signals. Thus, the combination of PCB and PTT can emerge as a translational modality for retinoblastoma therapy.

更新日期：2022-01-10

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南