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Glucolipids and lipoteichoic acids affect the activity of SigI, an alternative sigma factor, and WalKR, an essential two-component system, in Bacillus subtilis
Genes to Cells ( IF 1.3 ) Pub Date : 2021-12-15 , DOI: 10.1111/gtc.12912 Satoshi Matsuoka 1 , Yoko Shimizu 1 , Kaori Nobe 1 , Kouji Matsumoto 1 , Kei Asai 1, 2 , Hiroshi Hara 1
Genes to Cells ( IF 1.3 ) Pub Date : 2021-12-15 , DOI: 10.1111/gtc.12912 Satoshi Matsuoka 1 , Yoko Shimizu 1 , Kaori Nobe 1 , Kouji Matsumoto 1 , Kei Asai 1, 2 , Hiroshi Hara 1
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In a Bacillus subtilis ugtP mutant lacking glucolipids, SigI was activated in the log phase, and the activation of SigI in the mutant was suppressed by the expression of native ugtP. By contrast, SigI was inhibited in a yfnI mutant lacking one of the lipoteichoic acid (LTA) synthase genes, and the inhibition was suppressed by the expression of yfnI. A series of mutation analyses of the sigI promoter revealed that the two WalR binding sites were involved in the increase of PsigI-lacZ activity in the ugtP mutant and decrease of the lacZ activity in the yfnI mutant. Transcription from the SigI recognition sequence was enhanced in the ugtP mutant, whereas yfnI disruption inhibited the transcription from the SigA recognition sequence in the sigI promoter. We found that not only SigI but also WalKR, the essential two-component system, was activated in the ugtP mutant and inhibited in the yfnI mutant. The walK mutants with activated WalR exhibited abnormal morphology, but this phenotype was suppressed by the addition of MgSO4. We conclude that glucolipids and LTA are key compounds in the maintenance of normal cell surface structure in B. subtilis.
中文翻译：
糖脂和脂磷壁酸影响枯草芽孢杆菌中 SigI（另一种 sigma 因子）和 WalKR（一种必需的双组分系统）的活性
在缺乏糖脂的枯草芽孢杆菌ugtP突变体中，SigI在对数期被激活，并且突变体中SigI的激活被天然ugtP的表达抑制。相比之下，SigI 在缺乏一种脂磷壁酸 (LTA) 合酶基因的yfnI突变体中受到抑制，并且抑制被yfnI的表达抑制。对sigI启动子的一系列突变分析表明，这两个WalR结合位点参与了ugtP突变体中P sigI - lacZ活性的增加和yfnI中lacZ活性的降低。突变体。SigI 识别序列的转录在ugtP突变体中增强，而yfnI中断抑制了sigI启动子中 SigA 识别序列的转录。我们发现不仅 SigI，而且必要的双组分系统 WalKR 在 ugtP 突变体中被激活并在yfnI突变体中被抑制。具有活化 WalR的walK突变体表现出异常的形态，但这种表型被添加 MgSO 4抑制。我们得出结论，糖脂和 LTA 是维持B中正常细胞表面结构的关键化合物。枯草芽孢杆菌。
更新日期：2021-12-15
中文翻译：
糖脂和脂磷壁酸影响枯草芽孢杆菌中 SigI（另一种 sigma 因子）和 WalKR（一种必需的双组分系统）的活性
在缺乏糖脂的枯草芽孢杆菌ugtP突变体中，SigI在对数期被激活，并且突变体中SigI的激活被天然ugtP的表达抑制。相比之下，SigI 在缺乏一种脂磷壁酸 (LTA) 合酶基因的yfnI突变体中受到抑制，并且抑制被yfnI的表达抑制。对sigI启动子的一系列突变分析表明，这两个WalR结合位点参与了ugtP突变体中P sigI - lacZ活性的增加和yfnI中lacZ活性的降低。突变体。SigI 识别序列的转录在ugtP突变体中增强，而yfnI中断抑制了sigI启动子中 SigA 识别序列的转录。我们发现不仅 SigI，而且必要的双组分系统 WalKR 在 ugtP 突变体中被激活并在yfnI突变体中被抑制。具有活化 WalR的walK突变体表现出异常的形态，但这种表型被添加 MgSO 4抑制。我们得出结论，糖脂和 LTA 是维持B中正常细胞表面结构的关键化合物。枯草芽孢杆菌。
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