A pyridine-dicarbohydrazide-based chemosensor TMC (N², N⁶-bis((E)-5-chloro-2-hydroxybenzylidene)pyridine-2,6-dicarbohydrazide) showed a marked fluorescence response with Al³⁺. With analysis of Job plot and electrospray ionization (ESI)-mass spectrometry, the association of TMC to Al³⁺ was determined to be a 1:1 ratio. Detection limit of TMC for Al³⁺ was 1.56 μM. Especially, TMC could distinctly distinguish Al³⁺ from Ga³⁺ and In³⁺. TMC could suitably analyze Al³⁺ in a real environment with great recovery and relative standard deviation (RSD). In addition, TMC-coated test kits can be practically used for recognition of Al³⁺. Binding process of TMC to Al³⁺ was illustrated with density functional theory (DFT) calculations, NMR titration, ESI-mass, and UV–vis experiments.

中文翻译：

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一种基于吡啶-二碳酰肼的荧光开启检测Al3+的化学传感器

基于吡啶-二碳酰肼的化学传感器TMC (N ² , N ⁶ -bis ((E)-5-chloro-2-hydroxybenzylidene)pyridine-2,6-dicarbohydrazide) 对 Al ³⁺显示出显着的荧光响应。通过工作图和电喷雾电离 (ESI)-质谱分析，确定TMC与 Al ³⁺的关联为1:1。TMC对Al ³⁺的检测限为1.56 μM。特别是TMC可以明显地区分Al ³⁺与Ga ³⁺和In ³⁺。TMC可以适当地分析 Al ³⁺在具有良好恢复和相对标准偏差 (RSD) 的真实环境中。此外，TMC涂层检测试剂盒可实际用于识别 Al ³⁺。TMC与 Al ³⁺的结合过程通过密度泛函理论 (DFT) 计算、NMR 滴定、ESI 质量和 UV-vis 实验进行了说明。