Integrative Analysis of the Gut Microbiota and Metabolome for In Vitro Human Gut Fermentation Modeling,Journal of Agricultural and Food Chemistry

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Integrative Analysis of the Gut Microbiota and Metabolome for In Vitro Human Gut Fermentation Modeling
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-12-10 , DOI: 10.1021/acs.jafc.1c04259
Chu Wan ₁ , Kaizhang Wu ₁ , Xingyu Lu ₁ , Fang Fang ₁ , Yaqian Li ₁ , Yumin Zhao ₁ , Shubo Li ₁ , Jie Gao ₁

Affiliation

This study aimed to find the best in vitro fermentation method by integrative analysis of the gut microbiota and metabolome. We selected five different media: brain heart infusion broth, Luria–Bertani broth, Mueller–Hinton broth, anaerobe basal broth, and anaerobic medium base (AMB). After in vitro fermentation, the gut microbiota and metabolites were analyzed at different culture times. The results showed that different culture media have different effects on the bacterial community structure and metabolites. The integrative analysis of gut microbiota and metabolism also proved that AMB medium is effective in keeping a stable bacterial community structure and producing less metabolites and short-chain fatty acids by simulating the nutrient-poor microenvironment in the human gut during in vitro fermentation. Thus, culturing with AMB medium for 48 h is the most suitable in vitro model for human gut microbiota fermentation, which provides an alternative approach for diet and health research.

中文翻译：

用于体外人类肠道发酵建模的肠道微生物群和代谢组的综合分析

本研究旨在通过对肠道微生物群和代谢组的综合分析，寻找最佳的体外发酵方法。我们选择了五种不同的培养基：脑心浸液肉汤、Luria-Bertani 肉汤、Mueller-Hinton 肉汤、厌氧菌基础肉汤和厌氧培养基 (AMB)。后在体外在发酵过程中，分析了不同培养时间的肠道微生物群和代谢物。结果表明，不同培养基对细菌群落结构和代谢物的影响不同。肠道微生物群和代谢的综合分析还证明，AMB 培养基通过模拟体外发酵过程中人体肠道中营养贫乏的微环境，可有效保持稳定的细菌群落结构并产生较少的代谢物和短链脂肪酸。因此，用 AMB 培养基培养 48 小时是最适合人体肠道微生物群发酵的体外模型，为饮食和健康研究提供了一种替代方法。

更新日期：2021-12-22

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