Real-Time In Situ Sequential Fluorescence Activation Imaging of Cyt c and Caspase-9 with a Gold–Selenium-Bonded Nanoprobe,Analytical Chemistry

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Real-Time In Situ Sequential Fluorescence Activation Imaging of Cyt c and Caspase-9 with a Gold–Selenium-Bonded Nanoprobe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-12-09 , DOI: 10.1021/acs.analchem.1c03872
Bo Liu ₁ , Jingjing Li ₁ , Ping Zhou ₁ , Wei Pan ₁ , Na Li ₁ , Bo Tang ₁

Affiliation

Apoptosis, as a very important mode of programmed death, is closely associated with many diseases. Real-time in situ monitoring of the dynamic change of the apoptotic process remains a great challenge. Herein, a nanoprobe based on the gold–selenium (Au–Se) bond was developed for a sequential fluorescence activation imaging of cytochrome c (Cyt c) and caspase-9, two important apoptotic signaling molecules, to monitor the progression of apoptosis. The Cyt c aptamer and caspase-9-cleavable peptide chains labeled with two dyes were modified onto the surface of gold nanoparticles (Au NPs) by the Au–Se bond, which can be activated by upstream Cyt c and downstream caspase-9 to trigger fluorescence recovery. The Au–Se nanoprobe exhibited good specificity and stability. Compared with the traditional nanoprobe based on the gold–sulfur (Au–S) bond, the interference of biological thiols on the Au–Se nanoprobe can be effectively avoided. Importantly, the Au–Se nanoprobe can image the sequential changes of the two markers in situ in real time during cell apoptosis. This work provides an effective tool for the accurate and real-time detection of apoptosis and is conducive to the in-depth study of the relationship between apoptosis and disease.

中文翻译：

使用金-硒键合纳米探针对 Cyt c 和 Caspase-9 进行实时原位序列荧光激活成像

细胞凋亡作为一种非常重要的程序性死亡方式，与多种疾病密切相关。实时原位监测细胞凋亡过程的动态变化仍然是一个巨大的挑战。在此，开发了一种基于金-硒 (Au-Se) 键的纳米探针，用于细胞色素 c (Cyt c ) 和 caspase-9（两种重要的凋亡信号分子）的连续荧光激活成像，以监测细胞凋亡的进展。细胞色素c ^适体和caspase-9的可裂解肽链标记有两种染料被修改到的金纳米粒子（金纳米粒子）通过的Au-Se键的表面，其可以通过细胞色素上游激活Ç和下游 caspase-9 触发荧光恢复。Au-Se纳米探针表现出良好的特异性和稳定性。与基于金-硫（Au-S）键的传统纳米探针相比，可以有效避免生物硫醇对Au-Se纳米探针的干扰。重要的是，Au-Se 纳米探针可以在细胞凋亡过程中实时对两种标记物的连续变化进行原位成像。该工作为准确、实时检测细胞凋亡提供了有效工具，有利于深入研究细胞凋亡与疾病的关系。

更新日期：2021-12-21

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