Lysine β-Hydroxybutyrylation Improves Stability of COVID-19 Antibody,Biomacromolecules

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Lysine β-Hydroxybutyrylation Improves Stability of COVID-19 Antibody
Biomacromolecules ( IF 5.5 ) Pub Date : 2021-12-08 , DOI: 10.1021/acs.biomac.1c01435
Zihua Li ₁ , Yudian Zhang ₂ , Meng Han ₃ , Haiteng Deng ₂ , Fuqing Wu ₂ , Gang Liu ₁ , Guo-Qiang Chen _{2,

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Affiliation

β-Hydroxybutyrate (3HB) is a small molecule produced as a ketone body in mammalian animals. It has been found that 3HB provides not only energy for a body, it also participates in cell signal transduction events as a signal molecule. This study focuses on investigation of 3HB immunomodulatory mechanisms. Proteomic analysis indicates a new post-translational modification of β-hydroxybutyrylation (Kbhb) on antibodies. Because of the low level of Kbhb antibodies and the associated difficulty in purifying them, simulated Kbhb antibody was produced using chemical modification in vitro. The chemically modified Kbhb antibody was shown to improve the stability of antibodies to protease and heat treatments. Furthermore, Kbhb of antibodies stabilizes the antibodies in plasma. As a remarkable example, COVID-19 neutralizing antibody B38 produced by 293T cells was Kbhb modified and stabilized in vivo, providing a strategy for the possibility of extending the protection effects of COVID-19 antibodies.

中文翻译：

赖氨酸 β-羟基丁酰化可提高 COVID-19 抗体的稳定性

β-羟基丁酸酯 (3HB) 是一种在哺乳动物中以酮体形式产生的小分子。已经发现，3HB不仅为身体提供能量，还作为信号分子参与细胞信号转导事件。本研究的重点是研究 3HB 免疫调节机制。蛋白质组学分析表明抗体上的 β-羟基丁酰化 (Kbhb) 存在新的翻译后修饰。由于 Kbhb 抗体水平低且难以纯化，因此采用体外化学修饰生产模拟 Kbhb 抗体. 经化学修饰的 Kbhb 抗体显示可提高抗体对蛋白酶和热处理的稳定性。此外，抗体的 Kbhb 使血浆中的抗体稳定。作为一个显着的例子，由 293T 细胞产生的 COVID-19 中和抗体 B38在体内经过 Kbhb 修饰和稳定化，为扩展 COVID-19 抗体的保护作用提供了可能的策略。

更新日期：2022-01-10

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