Theaflavin 3, 3-Digallate 通过改善卵母细胞质量和调节颗粒细胞功能延缓卵巢衰老,Oxidative Medicine and Cellular Longevity

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Theaflavin 3, 3-Digallate 通过改善卵母细胞质量和调节颗粒细胞功能延缓卵巢衰老
Oxidative Medicine and Cellular Longevity Pub Date : 2021-12-08 , DOI: 10.1155/2021/7064179
Jiahuan He _{1,

2} , Guidong Yao _{1,

2} , Qina He _{1,

2} , Tongwei Zhang _{1,

2} , Huiying Fan _{1,

2} , Yucheng Bai _{1,

2} , Junya Zhang _{1,

2} , Guang Yang _{1,

2} , Ziwen Xu _{1,

2} , Jingyi Hu _{1,

2} , Yingpu Sun _{1,

2}

Affiliation

卵巢衰老是指卵巢功能随着生理年龄的增加而逐渐衰退，表现为卵巢储备减少、衰老相关标志物升高、卵母细胞质量下降等。随着女性生育能力的下降和人口老龄化的加剧，延缓卵巢衰老以保持生育能力，提高女性的生活质量迫在眉睫。茶黄素 3, 3 -二没食子酸酯（TF3）是一种从红茶中提取的具有天然生物活性的多酚类化合物，其抗氧化特性对维持人体健康和延缓衰老具有重要作用；然而，TF3对女性生殖和卵巢功能的影响尚不清楚。在这里，我们表明 TF3 可以保留原始卵泡池，部分恢复发情周期，并增加老年小鼠的后代数量。同时，TF3灌胃增加了取卵数，降低了活性氧水平，增加了谷胱甘肽水平，降低了诱导排卵后卵母细胞纺锤体的异常率。此外，TF3抑制人颗粒细胞凋亡并提高其抗氧化应激能力。高通量测序和小分子靶向药理学预测表明，TF3影响多种途径和基因表达水平，主要涉及生殖和发育过程。它还可能通过靶向mTOR来调节自噬途径来影响细胞功能，从而延缓卵巢衰老的过程。该研究表明，TF3可作为潜在的膳食补充剂，保护卵巢功能免受衰老，从而提高高龄女性的生活质量。

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Theaflavin 3, 3-Digallate Delays Ovarian Aging by Improving Oocyte Quality and Regulating Granulosa Cell Function

Ovarian aging refers to the gradual decline of ovarian function with increasing physiological age, manifested as decreased ovarian reserve, elevated aging-related markers, and reduced oocyte quality. With a declining female fertility and a growing aging population, it is urgent to delay ovarian aging to maintain fertility and improve the life quality of women. Theaflavin 3, 3-digallate (TF3) is a naturally bioactive polyphenol compound extracted from black tea, and its antioxidant properties play an important role in maintaining human health and delaying aging; however, the effects of TF3 on female reproduction and ovarian function are not yet clear. Here, we show that TF3 can preserve primordial follicle pool, partially restore the estrous cycle, and increase the offspring number of aged mice. Meanwhile, TF3 gavage increased the number of oocytes retrieved, decreased the level of reactive oxygen species, increased the level of glutathione, and decreased the abnormal rate of oocyte spindle after ovulation induction. Moreover, TF3 inhibited human granulosa cell apoptosis and improved their antioxidative stress ability. High-throughput sequencing and small-molecule-targeted pharmacological prediction show that TF3 affects multiple pathways and gene expression levels, mainly involved in reproductive and developmental processes. It may also affect cellular function by targeting mTOR to regulate the autophagic pathway, thereby delaying the process of ovarian aging. This study shows that TF3 can be used as a potential dietary supplement to protect ovary function from aging and thereby improving the life quality of advanced-age women.

更新日期：2021-12-08

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