用软骨细胞膜包裹的富含软骨细胞分泌组的微粒，以促进骨髓间充质干细胞的软骨形成并减少肥大,Journal of Materials Chemistry B

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用软骨细胞膜包裹的富含软骨细胞分泌组的微粒，以促进骨髓间充质干细胞的软骨形成并减少肥大
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2021-11-26 , DOI: 10.1039/d1tb02319e
Zizhao Tang _{1,

2} , Yan Lu _{1,

2} , Shixin Zhang _{1,

2} , Jing Wang ₃ , Qiguang Wang _{1,

2} , Yumei Xiao _{1,

2} , Xingdong Zhang _{1,

2}

Affiliation

软骨细胞与间充质干细胞（MSCs）的共培养是刺激MSCs软骨形成和减少肥大的有效方法，但供体部位供应有限和两阶段手术的要求是使用自体软骨细胞的主要障碍之一。临床设置。最近有证据表明上述共培养的软骨形成作用主要在于旁分泌，并且细胞膜在软骨细胞-MSC相互作用中也起着至关重要的作用，我们制造了一种多功能的“人造软骨细胞”设计，包括软骨细胞分泌组富集 PLGA 微粒，包裹软骨细胞膜碎片。人造软骨细胞显示出与天然软骨细胞相似的直径和表面电荷，在体内。因此，人工软骨细胞和骨髓间充质干细胞的共培养研究显示了软骨细胞分泌组和膜片段的有益作用，这也协同促进了细胞增殖、软骨基因表达、软骨基质的产生，并减少了体外表型肥大和在体内。总之，这项研究成功地开发了“人工软骨细胞”的概念验证设计，它可能克服使用天然软骨细胞的许多主要障碍，并为当前关节软骨功能再生的治疗策略提供了一种新的合成细胞方法.

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Chondrocyte secretome enriched microparticles encapsulated with the chondrocyte membrane to facilitate the chondrogenesis of BMSCs and reduce hypertrophy

Co-culture of chondrocytes and mesenchymal stem cells (MSCs) represents an effective way to stimulate the chondrogenesis of MSCs and reduce hypertrophy, but the limited donor site supply and the requirement of two-stage operations are among the major barriers of using autologous chondrocytes in clinical settings. With recent evidence indicating that the chondrogenic effects of the above co-culture mainly lied on the paracrine secretion, and that cell membranes also played crucial roles during the chondrocyte-MSC interaction, we fabricated a multifunctional design of “artificial chondrocytes”, which consist of chondrocyte secretome enriched PLGA microparticles with the encapsulation of chondrocytes’ membrane fragments. The artificial chondrocytes had shown a similar diameter and surface electrical charge to natural chondrocytes, with the preserved key chondrocyte membrane surface proteins and sustainedly released chondrogenic cytokines from the chondrocyte secretome to extend their effects in vivo. Consequently, the co-culture studies of artificial chondrocytes and bone marrow MSCs had shown the beneficial effects from both chondrocyte secretome and membrane fragments, which also synergistically facilitated the cell proliferation, chondrogenic gene expression, cartilaginous matrix production, and reduced phenotypic hypertrophy in vitro and in vivo. Together, this study has successfully developed the proof-of-concept design of “artificial chondrocytes”, which could potentially conquer many major barriers of using natural chondrocytes and provided a novel synthetic-cell approach to current therapeutical strategies towards the functional regeneration of articular cartilage.

更新日期：2021-12-07

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