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Dissolution-enhanced emission of 1,3,6,8-tetrakis(p-benzoic acid)pyrene for selectively detecting protamine and “on-to-on” heparin detection in water
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2021-11-22 , DOI: 10.1039/d1nj03946f
Hongtao Li 1 , Yuting Zhang 1 , Yan Huang 1 , Dapeng Cao 1 , Shitao Wang 1
Affiliation  

We developed a small organic molecule, 1,3,6,8-tetrakis(p-benzoic acid)pyrene (TBAPy), as a facile turn-on fluorescence probe for selectively detecting the basic protein protamine in water with a low detection limit of 24.14 ng mL−1. The TBAPy–protamine system could be further used as an “on-to-on” probe to detect the heparin protein with great fluorescence enhancement and simultaneous color change from green to blue. It can be explained by dissolution-enhanced emission (DEE) that the insoluble TBAPy probe is deprotonated to form the anionic species and dissolve in water, which suppresses the aggregation-caused quenching by electrostatic repulsion interactions. This DEE phenomenon is different from the widely reported aggregation-induced emission (AIE). These facile fluorescence “turn-on” and “on-to-on” methods for protein detection suggest that the DEE phenomenon is a very useful strategy for biosensing and can be potentially applied in food safety, disease diagnosis and healthcare.

中文翻译:

1,3,6,8-四(对苯甲酸)芘的溶解增强发射,用于选择性检测水中的鱼精蛋白和“on-to-on”肝素检测

我们开发了一种有机小分子 1,3,6,8-四(苯甲酸)芘 (TBAPy),作为一种简便的开启荧光探针,用于选择性检测水中的碱性蛋白鱼精蛋白,检测限低24.14 纳克·毫升-1. TBAPy-鱼精蛋白系统可以进一步用作“on-to-on”探针来检测肝素蛋白,具有很强的荧光增强和从绿色到蓝色的同步颜色变化。可以通过溶解增强发射 (DEE) 来解释不溶性 TBAPy 探针去质子化形成阴离子物质并溶解在水中,这抑制了由静电排斥相互作用引起的聚集引起的猝灭。这种 DEE 现象不同于广泛报道的聚集诱导发射 (AIE)。这些用于蛋白质检测的简便的荧光“开启”和“开启”方法表明 DEE 现象是一种非常有用的生物传感策略,可潜在地应用于食品安全、疾病诊断和医疗保健。
更新日期:2021-12-07
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