Sec3 exocyst 成分敲低抑制大脑皮层发育过程中的轴突形成和皮层神经元迁移,Journal of Neurochemistry

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Sec3 exocyst 成分敲低抑制大脑皮层发育过程中的轴突形成和皮层神经元迁移
Journal of Neurochemistry ( IF 4.2 ) Pub Date : 2021-12-04 , DOI: 10.1111/jnc.15554
Florentyna Bustos Plonka ₁ , Lucas J Sosa ₁ , Santiago Quiroga ₁

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神经元是已知最大的细胞，具有复杂且高度极化的形态，由一个细胞体 (soma)、几个树突和一个轴突组成。极性的建立需要初始的轴突生长，同时向轴突的质膜添加新膜。轴突扩张通过主要在神经元生长锥膜上的质膜前体囊泡的胞吐作用而发生。多蛋白外囊复合物驱动质膜囊泡融合的空间定位和特异性。然而，其不同蛋白质对神经元分化的特异性参与尚未完全确定。在目前的工作中，我们分析了 Sec3 的作用，Sec3 是一种突出的外囊复合蛋白对神经元分化的影响。使用小鼠海马原代培养物，我们确定 Sec3 在神经元极化之前的早期阶段在神经元中表达。此外，我们确定在培养的小鼠海马神经元中 Sec3 的沉默排除了极化。此外，使用子宫内电穿孔实验，我们确定 Sec3 敲低影响新皮质形成过程中的皮质神经元迁移和形态。我们的研究结果表明，外囊复合蛋白 Sec3 在神经元分化中的轴突形成和皮层发育过程中神经元祖细胞的迁移中起重要作用。我们确定 Sec3 敲低影响了新皮质形成过程中的皮质神经元迁移和形态。我们的研究结果表明，外囊复合蛋白 Sec3 在神经元分化中的轴突形成和皮层发育过程中神经元祖细胞的迁移中起重要作用。我们确定 Sec3 敲低影响了新皮质形成过程中的皮质神经元迁移和形态。我们的研究结果表明，外囊复合蛋白 Sec3 在神经元分化中的轴突形成和皮层发育过程中神经元祖细胞的迁移中起重要作用。

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Sec3 exocyst component knockdown inhibits axonal formation and cortical neuronal migration during brain cortex development

Neurons are the largest known cells, with complex and highly polarized morphologies and consist of a cell body (soma), several dendrites, and a single axon. The establishment of polarity necessitates initial axonal outgrowth in concomitance with the addition of new membrane to the axon's plasmalemma. Axolemmal expansion occurs by exocytosis of plasmalemmal precursor vesicles primarily at the neuronal growth cone membrane. The multiprotein exocyst complex drives spatial location and specificity of vesicle fusion at plasma membrane. However, the specific participation of its different proteins on neuronal differentiation has not been fully established. In the present work we analyzed the role of Sec3, a prominent exocyst complex protein on neuronal differentiation. Using mice hippocampal primary cultures, we determined that Sec3 is expressed in neurons at early stages prior to neuronal polarization. Furthermore, we determined that silencing of Sec3 in mice hippocampal neurons in culture precluded polarization. Moreover, using in utero electroporation experiments, we determined that Sec3 knockdown affected cortical neurons migration and morphology during neocortex formation. Our results demonstrate that the exocyst complex protein Sec3 plays an important role in axon formation in neuronal differentiation and the migration of neuronal progenitors during cortex development.

更新日期：2021-12-09

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