2,4,6-Trinitrophenol (TNP) has been widely used for a long time. The adverse effects of TNP on ecological environment and human health have promoted researchers to develop various methods for detecting TNP. Among multifarious technologies utilized for the TNP detection, fluorescence strategy based on different functional materials has become an effective and efficient method attributed to its merits such as preferable sensitivity and selectivity, rapid response speed, simple operation, and lower cost, which is also the focus of review. This review summarizes the development status of fluorescence sensors for TNP in a detailed and systematic way, especially focusing on the research progress since 2015. The sensing properties of fluorescent materials for TNP are the core of this review, including nanomaterials, organic small molecules, emerging supramolecular systems, aggregation induced emission materials and others. Moreover, the development direction and prospect of fluorescence sensing method in the field of TNP detection are introduced and discussed at the end of review.