In this paper, the room temperature hydrothermal synthesis method was used instead of the traditional solvothermal synthesis method to successfully prepare Cu-BTC, the growth of Cu-BTC was studied, and it was successfully applied to SCR flue gas denitration. The physic-chemical properties of catalyst samples were characterized by multiple techniques, such as SEM, XRD, FTIR, XPS and BET. The results showed that: (1) Cu-BTC prepared at different reaction times maintained its unique hexagonal prism crystal structure. As the reaction time increased, the purity and specific surface area of Cu-BTC gradually increased, up to 1521.320 m²/g. (2) Cu-BTC-30 h catalyst had a high O_α/(O_α+O_ß) ratio and could act as an electron acceptor to react with NO. The surface of the catalyst contained two valence state (Cu²⁺ and Cu⁺), and Cu²⁺ was dominant. (3) As the preparation time of the catalyst increased, the denitration also increased. When the preparation time was 30 h, the denitration rate reached the highest at 270 °C, which was as high as 90.36%.