Microchimica Acta ( IF 5.3 ) Pub Date : 2021-11-27 , DOI: 10.1007/s00604-021-05107-2 Mohammad Mehmandoust 1, 2 , Yasamin Khoshnavaz 1 , Mustafa Tuzen 3, 4 , Nevin Erk 1, 2
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A novel and sensitive voltammetric nanosensor was developed for the first time for trace level monitoring of favipiravir based on gold/silver core–shell nanoparticles (Au@Ag CSNPs) with conductive polymer poly (3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) and functionalized multi carbon nanotubes (F-MWCNTs) on a glassy carbon electrode (GCE). The formation of Au@Ag CSNPs/PEDOT:PSS/F-MWCNT composite was confirmed by various analytical techniques, including X-ray diffraction (XRD), ultraviolet–visible spectroscopy (UV–Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and field-emission scanning electron microscopy (SEM). Under the optimized conditions and at a typical working potential of + 1.23 V (vs. Ag/AgCl), the Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE revealed linear quantitative ranges from 0.005 to 0.009 and 0.009 to 1.95 µM with a limit of detection 0.46 nM (S/N = 3) with acceptable relative standard deviations (1.1-4.9 %) for pharmaceutical formulations, urine, and human plasma samples without applying any sample pretreatment (1.12–4.93%). The interference effect of antiviral drugs, biological compounds, and amino acids was negligible, and the sensing system demonstrated outstanding reproducibility, repeatability, stability, and reusability. The findings revealed that this assay strategy has promising applications in diagnosing FAV in clinical samples, which could be attributed to the large surface area on active sites and high conductivity of bimetallic nanocomposite.
Graphical abstract
中文翻译：
基于双金属纳米复合材料的伏安传感器测定法匹拉韦作为抗病毒药物
基于金/银核壳纳米粒子（Au@Ag CSNPs）和导电聚合物聚（3,4-乙烯二氧噻吩）聚苯乙烯磺酸盐（PEDOT： PSS）和玻璃碳电极（GCE）上的功能化多碳纳米管（F-MWCNT）。Au@Ag CSNPs/PEDOT:PSS/F-MWCNT复合材料的形成通过各种分析技术得到证实，包括X射线衍射（XRD）、紫外-可见光谱（UV-Vis）、透射电子显微镜（TEM）、能量-色散 X 射线光谱 (EDX) 和场发射扫描电子显微镜 (SEM)。在优化条件和 + 1.23 V（相对于 Ag/AgCl）的典型工作电位下，Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE 显示出从 0 开始的线性定量范围。005 至 0.009 和 0.009 至 1.95 µM，检测限为 0.46 nM (S/N = 3)，药物制剂、尿液和人血浆样品的相对标准偏差 (1.1-4.9 %) 可接受，无需进行任何样品预处理 (1.12 –4.93%）。抗病毒药物、生物化合物和氨基酸的干扰效应可忽略不计，传感系统表现出出色的重现性、重复性、稳定性和可重复使用性。研究结果表明，这种测定策略在诊断临床样品中的 FAV 方面具有广阔的应用前景，这可能归因于双金属纳米复合材料的活性位点的大表面积和高电导率。未应用任何样品预处理的尿液和人血浆样品 (1.12–4.93%)。抗病毒药物、生物化合物和氨基酸的干扰效应可忽略不计，传感系统表现出出色的重现性、重复性、稳定性和可重复使用性。研究结果表明，这种测定策略在诊断临床样品中的 FAV 方面具有广阔的应用前景，这可能归因于双金属纳米复合材料的活性位点的大表面积和高电导率。未应用任何样品预处理的尿液和人血浆样品 (1.12–4.93%)。抗病毒药物、生物化合物和氨基酸的干扰效应可忽略不计，传感系统表现出出色的重现性、重复性、稳定性和可重复使用性。研究结果表明，这种测定策略在诊断临床样品中的 FAV 方面具有广阔的应用前景，这可能归因于双金属纳米复合材料的活性位点的大表面积和高电导率。
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