促进人类口腔微生物群落研究的体外生物膜模型系统,Letters in Applied Microbiology

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促进人类口腔微生物群落研究的体外生物膜模型系统
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-11-26 , DOI: 10.1111/lam.13618
S-Q An _{1,

2} , R Hull _{1,

2,

3} , A Metris ₄ , P Barrett ₄ , J S Webb _{1,

2} , P Stoodley _{2,

5,

6,

7}

Affiliation

人类口腔是多种微生物群的宿主。关于口腔微生物行为的大部分知识来自对单个或几个栽培物种的研究，这些情况并不能完全反映更复杂的微生物群或多物种生物膜中生物体的功能。允许检查生物膜在口腔定植过程中所起的作用的验证模型的数量也有限。CDC 生物膜反应器是一种标准方法，已被用于研究人类微生物群成员之间的相互作用，从而使研究能够在营养供应和废物冲刷得到控制的条件下在较长时间内完成。这项工作的目的是开发一种强大的体外来自汇集唾液接种物的生物膜模型系统，用于研究口腔微生物群的发育、可重复性和稳定性。通过对 16S rRNA 基因可变区的深度测序，我们发现 CDC 生物膜反应器可用于有效培养包含先前被确定为核心唾液微生物群的所有六个主要门的微生物群。经过一段适应期后，每个反应堆中的群落都趋于稳定。复制反应器主要由共享的核心微生物群组成；复制反应器之间的差异主要是由共享操作分类单元的丰度变化驱动的。我们得出结论，CDC 生物膜反应器可用于培养复制人类口腔关键特征的群落，并且是促进这些群落动态研究的有用工具。

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An in vitro biofilm model system to facilitate study of microbial communities of the human oral cavity

The human oral cavity is host to a diverse microbiota. Much of what is known about the behaviour of oral microbes derives from studies of individual or several cultivated species, situations which do not totally reflect the function of organisms within more complex microbiota or multispecies biofilms. The number of validated models that allow examination of the role that biofilms play during oral cavity colonization is also limited. The CDC biofilm reactor is a standard method that has been deployed to study interactions between members of human microbiotas allowing studies to be completed during an extended period under conditions where nutrient availability, and washout of waste products are controlled. The objective of this work was to develop a robust in vitro biofilm-model system from a pooled saliva inoculum to study the development, reproducibility and stability of the oral microbiota. By employing deep sequencing of the variable regions of the 16S rRNA gene, we found that the CDC biofilm reactor could be used to efficiently cultivate microbiota containing all six major phyla previously identified as the core saliva microbiota. After an acclimatisation period, communities in each reactor stabilised. Replicate reactors were predominately populated by a shared core microbiota; variation between replicate reactors was primarily driven by shifts in abundance of shared operational taxonomic units. We conclude that the CDC biofilm reactor can be used to cultivate communities that replicate key features of the human oral cavity and is a useful tool to facilitate studies of the dynamics of these communities.

更新日期：2021-11-26

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