Deep Sequencing of a Systematic Peptide Library Reveals Conformationally-Constrained Protein Interface Peptides that Disrupt a Protein-Protein Interaction,ChemBioChem

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Deep Sequencing of a Systematic Peptide Library Reveals Conformationally-Constrained Protein Interface Peptides that Disrupt a Protein-Protein Interaction
ChemBioChem ( IF 2.6 ) Pub Date : 2021-11-24 , DOI: 10.1002/cbic.202100504
David M Boragine ₁ , Wanzhi Huang ₂ , Lynn H Su ₂ , Timothy Palzkill ₂

Affiliation

Disrupting protein-protein interactions is difficult due to the large and flat interaction surfaces of the binding partners. We utilized high throughput oligonucleotide synthesis, peptide phage display library construction, affinity selection, and deep sequencing to show a peptide library encompassing a binding partner in a protein-protein interaction can serve as a source of peptides that mimic the interface and provide a starting point for developing inhibitors that block the interface.

中文翻译：

系统肽库的深度测序揭示了破坏蛋白质-蛋白质相互作用的构象受限蛋白质界面肽

由于结合配偶体的相互作用表面又大又平，破坏蛋白质-蛋白质相互作用很困难。我们利用高通量寡核苷酸合成、肽噬菌体展示库构建、亲和力选择和深度测序来证明包含蛋白质-蛋白质相互作用中的结合配偶体的肽库可以作为模拟界面的肽的来源并提供起点用于开发阻断界面的抑制剂。

更新日期：2021-11-24

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