The Acinetobacter indicus strain ZJB20129 isolated from an urban sewage treatment plant demonstrated the heterotrophic nitrification-aerobic denitrification (HN-AD) ability. Stain ZJB20129 could remove 98.73% of ammonium-N, 97.26% of nitrite-N and 96.55% of nitrate-N, and the maximum removal rate was 3.66, 4.62 and 5.21 mg/L/h, respectively. Ammonium was preferentially used during simultaneous nitrification and denitrification. Strain ZJB20129 exhibited highest ammonium removal capability when carbon source was sodium succinate, C/N ratio was 15, pH was 8.0, and temperature was 35 ℃. Key enzymes involved in HN-AD including hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase as well as their encoding genes were detected, and the metabolic pathway of HN-AD was subsequently predicted. Our results suggested that Acinetobacter indicus ZJB20129 displayed superior nitrogen removal performance on actual wastewater and thus made it have a good application prospect in wastewater biological treatment.

印度不动杆菌从城市污水处理厂分离的菌株ZJB20129表现出异养硝化-好氧反硝化（HN-AD）能力。污渍ZJB20129可去除98.73%的氨氮、97.26%的亚硝酸盐和96.55%的硝酸盐，最大去除率分别为3.66、4.62和5.21 mg/L/h。在同时硝化和反硝化过程中优先使用铵。菌株ZJB20129在碳源为丁二酸钠、C/N比为15、pH为8.0、温度为35 ℃时表现出最高的氨去除能力。检测了参与HN-AD的关键酶包括羟胺氧化酶、周质硝酸还原酶和亚硝酸还原酶及其编码基因，进而预测了HN-AD的代谢途径。我们的结果表明印度不动杆菌ZJB20129在实际废水中表现出优异的脱氮性能，在废水生物处理中具有良好的应用前景。