硅胶基聚五溴苯乙烯固定相在毛细管电色谱中的分离特性及性能评价,Analytical Methods

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硅胶基聚五溴苯乙烯固定相在毛细管电色谱中的分离特性及性能评价
Analytical Methods ( IF 2.7 ) Pub Date : 2021-11-04 , DOI: 10.1039/d1ay01594j
Zhihua Zhong ₁ , Zhanying Chu ₂ , Ziyi Dong ₁ , Weibing Zhang ₁ , Lingyi Zhang ₁

Affiliation

制备了填充有二氧化硅基聚（五溴苯乙烯）颗粒（表示为 SiO ₂ @pPBS）的混合模式毛细管柱，并将其应用于毛细管电色谱 (CEC) 分离。由于聚合物链中存在苯环和溴原子，SiO ₂@pPBS 色谱柱提供反相/亲水混合模式保留机制，这是由于固定相与各种分析物（包括烷基苯、多环芳烃、核苷、苯酚和苯胺）之间的亲水、疏水和 π-π 相互作用。在 CEC 模式下，带电溶质的分离行为不仅与与固定相的相互作用有关，还受到电泳效应的影响，这可能导致与高效液相色谱 (HPLC) 相比的选择性不同。对氯苯胺的柱效高达 1.22 × 10 ⁵ N m ^-1。此外，苯胺保留时间的 RSD 为 run to run ( n = 5), day to day ( n= 5) 和列到列 ( n = 3) 均小于 4.4%。最后将SiO ₂ @pPBS 毛细管柱应用于焦化废水的分离，取得了满意的效果。结果表明，RP/HILIC混合模式的SiO ₂ @pPBS毛细管填充柱具有很大的应用潜力。

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The separation characteristics and performance evaluation of the silica-based poly(pentabromostyrene) stationary phase in capillary electrochromatography

A mixed-mode capillary column packed with silica-based poly(pentabromostyrene) particles (denoted as SiO₂@pPBS) was prepared and applied to capillary electrochromatography (CEC) separation. With the presence of benzene rings and bromine atoms in polymer chains, the SiO₂@pPBS column provides a reversed-phase/hydrophilic mixed-mode retention mechanism owing to hydrophilic, hydrophobic and π–π interactions between the stationary phase and various analytes, including alkylbenzenes, polycyclic aromatic hydrocarbons, nucleosides, phenols and anilines. In CEC mode, the separation behavior of charged solutes is not only related to the interaction with the stationary phase, but also influenced by electrophoretic effects, which may lead to different selectivities compared to high performance liquid chromatography (HPLC). A column efficiency of up to 1.22 × 10⁵ N m⁻¹ was achieved for p-chloroaniline. Besides, the RSDs of retention time of anilines for run to run (n = 5), day to day (n = 5) and column to column (n = 3) were all less than 4.4%. Finally, the SiO₂@pPBS capillary column was applied to the separation of coking wastewater with satisfactory results. All the results demonstrated that the SiO₂@pPBS capillary packed column with RP/HILIC mixed-mode has great application potential.

更新日期：2021-11-24

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