Copper fumarate with high-bifunctional nanozyme activities at different pH values for glucose and epinephrine colorimetric detection in human serum,Analyst

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Copper fumarate with high-bifunctional nanozyme activities at different pH values for glucose and epinephrine colorimetric detection in human serum
Analyst ( IF 3.6 ) Pub Date : 2021-11-16 , DOI: 10.1039/d1an01817e
Meihui Ying _{1,

2,

3} , Guizeng Yang _{1,

3} , Yuanjie Xu _{1,

2} , Huiling Ye _{1,

3} , Xing Lin _{1,

2} , Yi Lu _{1,

2} , Haibo Pan _{1,

2,

3,

4} , Yang Bai ₄ , Min Du ₃

Affiliation

Metal–organic frameworks (MOFs) have attracted extensive attention for the development of colorimetric detection methods due to their ease of modification and high density of active sites. However, most of the reported colorimetric sensors based on MOFs show only a single nanozyme activity. Herein, the bifunctional enzyme activities of a hexagonal prism Cu MOF with fumaric acid as the ligand (Cu FMA), namely laccase-like activity under alkaline conditions (pH = 8) and peroxidase-like activity under acidic conditions (pH = 4), were verified. The specificity of Cu FMA at different pH values may be due to the presence of the Cu⁺ active center introduced by the weak reducibility of FMA. At pH = 8, Cu⁺ active centers are beneficial for dissociating the H–O bonds of phenolic compounds for the laccase system. In contrast, the dissociation of H–O is weakened at pH = 4, which prompts the breaking of the O–O bonds of H₂O₂ as a Fenton-like reaction for the peroxidase system. Based on the dual enzyme activities, Cu FMA sensors exhibit outstanding detectability for epinephrine and glucose with linear ranges of 2.7–54.6 μM and 0.01–0.8 mM and detection limits of 1.1 μM and 2.28 × 10⁻⁷ M, respectively. The Cu FMA colorimetric sensor can be applied for detecting and measuring glucose and epinephrine in human serum samples. This work paves the way for Cu MOFs to be used as the basis for rational regulation of the activity of dual nanozymes and for multifunctional applications under completely independent conditions.

中文翻译：

富马酸铜在不同 pH 值下具有高双功能纳米酶活性，用于人血清中葡萄糖和肾上腺素的比色检测

金属有机框架（MOFs）由于易于修饰和高活性位点密度，在比色检测方法的发展中引起了广泛的关注。然而，大多数报道的基于 MOF 的比色传感器仅显示单一的纳米酶活性。在此，以富马酸为配体的六棱柱Cu MOF（Cu FMA）的双功能酶活性，即碱性条件下（pH = 8）下的漆酶样活性和酸性条件下（pH = 4）下的过氧化物酶活性，被验证。Cu FMA 在不同 pH 值下的特异性可能是由于FMA 弱还原性引入的 Cu ⁺活性中心的存在。在 pH = 8 时，Cu ⁺活性中心有利于解离漆酶系统酚类化合物的 H-O 键。相比之下，H-O 的解离在 pH = 4 时减弱，这促使 H ₂ O ₂的 O-O 键断裂，作为过氧化物酶系统的类芬顿反应。基于双酶活性，Cu FMA 传感器对肾上腺素和葡萄糖表现出出色的可检测性，线性范围为 2.7-54.6 μM 和 0.01-0.8 mM，检测限为 1.1 μM 和 2.28 × 10 ^-7M，分别。Cu FMA 比色传感器可用于检测和测量人血清样品中的葡萄糖和肾上腺素。这项工作为 Cu MOF 用作合理调节双纳米酶活性的基础以及在完全独立条件下的多功能应用铺平了道路。

更新日期：2021-12-09

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